Dependence of Na+,K+-ATPase and electrogenic component of Em in cultured myotubes on cell fusion

Chaya Brodie, Asia Bak, S. R. Sampson

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Abstract

This study was undertaken in order to determine the relation among cell fusion, [3H]ouabain binding and the membrane potential (Em) of cultured rat skeletal muscle. The amount of ouabain bound and the Em both increased with age, the increases being most dramatic following fusion. Inhibition of fusion prevented the developmental increases in both properties of cultured muscle. After fusion, the size of the electrogenic of Em, determined by the increased in Em produced by ouabain within 5-10 min, increased independent of the age at which fusion occurred. It is concluded that the increase in Em with age depends on postfusion appearance and activity of Na,K-ATPase.

Original languageEnglish
Pages (from-to)384-386
Number of pages3
JournalBrain Research
Volume336
Issue number2
DOIs
StatePublished - 17 Jun 1985

Keywords

  • Na,K-ATPase
  • cell fusion
  • culture
  • electrogenic pump
  • membrane potential
  • skeletal muscle

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