Abstract
Dipole radiation patterns change when a fluorescent molecule comes close to the boundary between media of different refractive indices. Near-interface molecules emit mostly into the higher-index medium, predominantly around the critical angle. The radiation pattern encodes information about the emitter distance, orientation, and the refractive index of the embedding medium. Analyses of the supercritical angle fluorescence on pupil plane images can retrieve this information and have been applied both for refractometry with subcellular resolution and for the detection of metabolically active cancerous cells. In this issue of ACS Nano, Ferdman et al. employ this strategy in a label-free assay for detecting single bacteria, based on measuring the refractive-index change produced by bacterial growth in a fluorophore-coated microfluidic channel.
| Original language | English |
|---|---|
| Pages (from-to) | 11725-11730 |
| Number of pages | 6 |
| Journal | ACS Nano |
| Volume | 12 |
| Issue number | 12 |
| DOIs | |
| State | Published - 26 Dec 2018 |
Bibliographical note
Publisher Copyright:Copyright © 2018 American Chemical Society.
Funding
We thank Dan Axelrod for inspiring our work on TIRF and SAF. M.B. acknowledges financial support from the Chaire d’Excellence Junior University Sorbonne Paris Cite,́ USPC. Our collaborative research on TIRF and SAF is financed by a French−Israeli ImagiNano LIA grant (to A.S. and M.O.), the Agence Nationale de la Recherche (ANR-10-INSB-04-01, grands investissements FranceBioImaging, FBI, to M.O.), and the Université Paris Descartes (invited professorship during the academic year 2017−2018, to A.S.). The Oheim lab is a member of the C’nano IdF and Ecole de Neurosciences de Paris (ENP) excellence clusters for nanobiotechnology and neurosciences, respectively.
| Funders | Funder number |
|---|---|
| Federal Bureau of Investigation | |
| Agence Nationale de la Recherche | ANR-10-INSB-04-01 |
| Université Paris Descartes |
