Decoding the Information Contained in Fluorophore Radiation Patterns

Maia Brunstein, Adi Salomon, Martin Oheim

Research output: Contribution to journalReview articlepeer-review

6 Scopus citations

Abstract

Dipole radiation patterns change when a fluorescent molecule comes close to the boundary between media of different refractive indices. Near-interface molecules emit mostly into the higher-index medium, predominantly around the critical angle. The radiation pattern encodes information about the emitter distance, orientation, and the refractive index of the embedding medium. Analyses of the supercritical angle fluorescence on pupil plane images can retrieve this information and have been applied both for refractometry with subcellular resolution and for the detection of metabolically active cancerous cells. In this issue of ACS Nano, Ferdman et al. employ this strategy in a label-free assay for detecting single bacteria, based on measuring the refractive-index change produced by bacterial growth in a fluorophore-coated microfluidic channel.

Original languageEnglish
Pages (from-to)11725-11730
Number of pages6
JournalACS Nano
Volume12
Issue number12
DOIs
StatePublished - 26 Dec 2018

Bibliographical note

Publisher Copyright:
Copyright © 2018 American Chemical Society.

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