DE-52 and DE-53 cellulose microcarriers - I. Growth of primary and established anchorage-dependent cells

S. Reuveny; L. Silberstein; A. Shahar; E. Freeman; A. Mizrahi

doi:10.1007/BF02796400

DE-52 and DE-53 cellulose microcarriers - I. Growth of primary and established anchorage-dependent cells

S. Reuveny, L. Silberstein, A. Shahar, E. Freeman, A. Mizrahi

Israel Institute for Biological Research

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

DEAE cellulose anion exchangers having small ion exchange capacity (0.5 to 2.0 meq/g dry material) were tested for cell attachment kinetics and capacity to support growth anchorage-dependent cells. It was found that cells from established cell lines (BHK and MDCK) can grow to confluency on DEAE cellulose particles having exchange capacity of 1 and 2 meq/g dry materials, DE-52 and DE-53, respectively. On the other hand, chick embryo fibroblasts (primary cells) can grow only on DE-53 particles.

Original language	English
Pages (from-to)	92-98
Number of pages	7
Journal	In Vitro
Volume	18
Issue number	2
DOIs	https://doi.org/10.1007/BF02796400
State	Published - Feb 1982
Externally published	Yes

Keywords

anchorage-dependent cells
cell attachment
microcarriers

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10.1007/BF02796400

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abstract = "DEAE cellulose anion exchangers having small ion exchange capacity (0.5 to 2.0 meq/g dry material) were tested for cell attachment kinetics and capacity to support growth anchorage-dependent cells. It was found that cells from established cell lines (BHK and MDCK) can grow to confluency on DEAE cellulose particles having exchange capacity of 1 and 2 meq/g dry materials, DE-52 and DE-53, respectively. On the other hand, chick embryo fibroblasts (primary cells) can grow only on DE-53 particles.",

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AU - Reuveny, S.

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AU - Shahar, A.

AU - Freeman, E.

AU - Mizrahi, A.

PY - 1982/2

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AB - DEAE cellulose anion exchangers having small ion exchange capacity (0.5 to 2.0 meq/g dry material) were tested for cell attachment kinetics and capacity to support growth anchorage-dependent cells. It was found that cells from established cell lines (BHK and MDCK) can grow to confluency on DEAE cellulose particles having exchange capacity of 1 and 2 meq/g dry materials, DE-52 and DE-53, respectively. On the other hand, chick embryo fibroblasts (primary cells) can grow only on DE-53 particles.

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DE-52 and DE-53 cellulose microcarriers - I. Growth of primary and established anchorage-dependent cells

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Keywords

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