DE-52 and DE-53 cellulose microcarriers - I. Growth of primary and established anchorage-dependent cells

S. Reuveny, L. Silberstein, A. Shahar, E. Freeman, A. Mizrahi

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

DEAE cellulose anion exchangers having small ion exchange capacity (0.5 to 2.0 meq/g dry material) were tested for cell attachment kinetics and capacity to support growth anchorage-dependent cells. It was found that cells from established cell lines (BHK and MDCK) can grow to confluency on DEAE cellulose particles having exchange capacity of 1 and 2 meq/g dry materials, DE-52 and DE-53, respectively. On the other hand, chick embryo fibroblasts (primary cells) can grow only on DE-53 particles.

Original languageEnglish
Pages (from-to)92-98
Number of pages7
JournalIn Vitro
Volume18
Issue number2
DOIs
StatePublished - Feb 1982
Externally publishedYes

Keywords

  • anchorage-dependent cells
  • cell attachment
  • microcarriers

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