d-Poly(lactide) and LHRH decapeptide stereointeractions investigated by vibrational spectroscopy

Jianming Zhang, Ashgan Beshra, Abraham J. Domb, Yukihiro Ozaki

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10 Scopus citations

Abstract

The interactions between poly(d-lactic acid) (PDLA) and l-configured leuprolide (LHRH) to form heterocomplexes were investigated by a combination of infrared (IR), Raman and near infrared spectroscopy (NIR). It was found that an α crystal with 103 helical conformation of PDLA is formed in the PDLA/LHRH heterocomplexes with various LHRH loadings, whereas the secondary structures of LHRH in these heterocompelexes are greatly affected by the blend ratio of LHRH and PDLA. Based on the analysis of IR, Raman and NIR spectra of the various heterocomplexes, it is suggested that stereoselective Van der Waals interactions, consisting of interwined α helices of PDLA and LHRH, is responsible for the driving force of PDLA and LHRH stereocomplexation. To clarify the solid structure and the potential interaction in these heterocomplexes with various LHRH loadings, temperature-dependent IR spectral measurements were also employed. On the basis of the results presented, a model for the PDLA and LHRH stereointeraction process was proposed.

Original languageEnglish
Pages (from-to)3016-3027
Number of pages12
JournalEuropean Polymer Journal
Volume43
Issue number7
DOIs
StatePublished - Jul 2007
Externally publishedYes

Bibliographical note

Funding Information:
Jianming Zhang thanks the Japan Society for the Promotion of Science (JSPS) for financial support. This work was partially supported by “Open Research Center” project for private universities: matching fund subsidy from MEXT (Ministry of Education, Culture, Sports, Science and Technology), 2001–2005. This work was also supported by Kwansei–Gakuin University “Special Research” project, 2004–2008. This work was supported in part by grants from the Israel Science Foundation (ISF) and the US-Israel Bionational Foundation (BSF).

Funding

Jianming Zhang thanks the Japan Society for the Promotion of Science (JSPS) for financial support. This work was partially supported by “Open Research Center” project for private universities: matching fund subsidy from MEXT (Ministry of Education, Culture, Sports, Science and Technology), 2001–2005. This work was also supported by Kwansei–Gakuin University “Special Research” project, 2004–2008. This work was supported in part by grants from the Israel Science Foundation (ISF) and the US-Israel Bionational Foundation (BSF).

FundersFunder number
US-Israel Bionational Foundation
Kwansei Gakuin University
Japan Society for the Promotion of Science
Ministry of Education, Culture, Sports, Science and Technology
United States-Israel Binational Science Foundation
Israel Science Foundation

    Keywords

    • Heterocomplexes
    • IR spectral measurements
    • d-Lactic acid
    • l-Configured leuprolide

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