Cytosolic phospholipase A2 gene in human and rat: chromosomal localization and polymorphic markers

Agnes Tay, Jason S. Simon, Jeremy Squire, Ken Hamel, Howard J. Jacob, Karl Skorecki

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82 Scopus citations

Abstract

We report the chromosomal localization and a simple sequence repeat (SSR) in the cytosolic phospholipase A2 (cPLA2) gene in both human and rat. A (CA)18 repeat in the promoter of the rat gene wasdetermined to exhibit length polymorphism when analyzed using the polymerase chain reaction (PCR) in 19 different inbred rat strains. Genotyping for this marker in 234 F2 progeny of a SHR × BN intercross mapped the gene to rat chromosome 13. Using a PCR strategy, a fragment of the promoter for the human gene was isolated, and a (CA)18 repeat was identified. Since this marker displayed a low heterozygosity index, we also identified a mononucleotide repeat in the promoter for cPLA2 that displayed a polymorphism information content value of 0.76. The human gene was mapped using fluorescence in situ hybridization (FISH) to chromosome 1q25. Of interest, the gene encoding the enzyme prostaglandin-endoperoxide synthase 2 (cyclooxygenase-2), which acts on the arachidonic acid product of cPLA2, was previously localized to this same chromosomal region, raising the possibility of coordinate regulation. Identification of intragenic markers may facilitate studies of polymorphic variants of these genes as candidates for disorders in which perturbations of the eicosanoid cascade may play a role.

Original languageEnglish
Pages (from-to)138-141
Number of pages4
JournalGenomics
Volume26
Issue number1
DOIs
StatePublished - 1 Mar 1995
Externally publishedYes

Bibliographical note

Funding Information:
We thank Dr. J. Knopf and J. Clark, Genetics Institute (Cambridge, MA) for human cPLA2 plasmid, D. Brown and Dr. M. Trolliet for help with mapping in the rat, and Dr. B. Beatty (Department of Pathology, Hospital for Sick Children) for valuable discussion. SHR × BN F2 intercross progeny were kindly provided by Drs. M. Pra-venec, V. Kren, and T. Kurtz, UCSF (San Francisco, CA) and Dr. J. E. Krieger, USP (Sao Paulo, Brazil). The technical assistance of J. Jung, Y. K. Ng, and Z. M. Zhang is gratefully acknowledged. A.T. thanks Dr. P. Maxwell for assistance with screening of the human genomic library. A.T. was supported by a National University of Singapore Overseas Graduate Scholarship. This work was supported by grants from the Medical Research Council, Kidney Foundation, and National Cancer Institutes of Canada (K.S.) and Bristol-Myers Squibb (H.J.J., K.L.S.).

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