Cytokine release and expression induced by OmpA proteins from the Gram-negative anaerobes, Porphyromonas asaccharolytica and Bacteroides fragilis

Lana Magalashvili, Shirley Lazarovich, Izabella Pechatnikov, Hannah M. Wexler, Yeshayahu Nitzan

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

OmpA proteins from Gram-negative anaerobes Porphyromonas asaccharolytica and Bacteroides fragilis induced release and expression of IL-1α, tumor necrosis factor (TNF)-α, IFN-γ, IL-6, and IL-10 from murine splenocytes in vitro in a dose-dependent fashion. The release of the cytokines induced by B. fragilis Bf-OmpA was at much lower levels compared with P. asaccharolytica Omp-PA; Bf-OmpA did not induce release of IL-10. Omp-PA and Bf-OmpA were able to upregulate mRNA expression of the tested cytokines. The results obtained with refolded Bf-OmpA were similar to those with native Bf-OmpA. The data presented in this research demonstrate for the first time that Omps from anaerobic bacteria can induce the release of cytokines, suggesting that Omp-PA and Bf-OmpA may play important roles in the pathogenic processes of these bacteria.

Original languageEnglish
Pages (from-to)252-259
Number of pages8
JournalFEMS Immunology and Medical Microbiology
Volume53
Issue number2
DOIs
StatePublished - Jul 2008

Keywords

  • Bacteroides fragilis
  • Cytokine
  • OmpA
  • Porphyromonas asaccharolytica

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