TY - JOUR
T1 - Cytokine release and expression induced by OmpA proteins from the Gram-negative anaerobes, Porphyromonas asaccharolytica and Bacteroides fragilis
AU - Magalashvili, Lana
AU - Lazarovich, Shirley
AU - Pechatnikov, Izabella
AU - Wexler, Hannah M.
AU - Nitzan, Yeshayahu
PY - 2008/7
Y1 - 2008/7
N2 - OmpA proteins from Gram-negative anaerobes Porphyromonas asaccharolytica and Bacteroides fragilis induced release and expression of IL-1α, tumor necrosis factor (TNF)-α, IFN-γ, IL-6, and IL-10 from murine splenocytes in vitro in a dose-dependent fashion. The release of the cytokines induced by B. fragilis Bf-OmpA was at much lower levels compared with P. asaccharolytica Omp-PA; Bf-OmpA did not induce release of IL-10. Omp-PA and Bf-OmpA were able to upregulate mRNA expression of the tested cytokines. The results obtained with refolded Bf-OmpA were similar to those with native Bf-OmpA. The data presented in this research demonstrate for the first time that Omps from anaerobic bacteria can induce the release of cytokines, suggesting that Omp-PA and Bf-OmpA may play important roles in the pathogenic processes of these bacteria.
AB - OmpA proteins from Gram-negative anaerobes Porphyromonas asaccharolytica and Bacteroides fragilis induced release and expression of IL-1α, tumor necrosis factor (TNF)-α, IFN-γ, IL-6, and IL-10 from murine splenocytes in vitro in a dose-dependent fashion. The release of the cytokines induced by B. fragilis Bf-OmpA was at much lower levels compared with P. asaccharolytica Omp-PA; Bf-OmpA did not induce release of IL-10. Omp-PA and Bf-OmpA were able to upregulate mRNA expression of the tested cytokines. The results obtained with refolded Bf-OmpA were similar to those with native Bf-OmpA. The data presented in this research demonstrate for the first time that Omps from anaerobic bacteria can induce the release of cytokines, suggesting that Omp-PA and Bf-OmpA may play important roles in the pathogenic processes of these bacteria.
KW - Bacteroides fragilis
KW - Cytokine
KW - OmpA
KW - Porphyromonas asaccharolytica
UR - http://www.scopus.com/inward/record.url?scp=45149084717&partnerID=8YFLogxK
U2 - 10.1111/j.1574-695x.2008.00423.x
DO - 10.1111/j.1574-695x.2008.00423.x
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C2 - 18503547
AN - SCOPUS:45149084717
SN - 0928-8244
VL - 53
SP - 252
EP - 259
JO - FEMS Immunology and Medical Microbiology
JF - FEMS Immunology and Medical Microbiology
IS - 2
ER -