Cytokine expression of HIV-infected monocytes/macrophages at the single-cell level

W. Glienke; R. Esser; H. von Briesen; K. Schuster; S. Müller; R. Unger; R. Andreesen; H. J. Stutte; H. Rübsamen-Waigmann

doi:10.1016/s0923-2516(07)80022-7

Cytokine expression of HIV-infected monocytes/macrophages at the single-cell level

W. Glienke, R. Esser, H. von Briesen, K. Schuster, S. Müller, R. Unger, R. Andreesen, H. J. Stutte, H. Rübsamen-Waigmann

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Monocytes of healthy donors were infected with HIV1 in vitro: 14-21 days after infection 50-70 % of the cells produced p24 HIV1 antigen as detected with anti-p24 immunostaining; infected cultures showed enhanced secretion of interleukin-6 (IL6), interleukin-8 (IL8) and tumour necrosis factor alpha (TNF-alpha). The expression of cytokines on the single-cell level was further analysed by in situ hybridization using non-radioactive digoxigenin for detection. HIV1 (p24⁺)-producing cells were compared with non-HIV (p24^-) -producing cells. All morphological subtypes of macrophages showed HIV production; no difference in cytokine expression was observed. Immunocytochemistry of HIV-infected and uninfected cultures also showed no difference in the pattern of IL1-beta, IL6, IL8 and TNF-alpha protein expression in the cells.

Original language	English
Pages (from-to)	193-197
Number of pages	5
Journal	Research in Virology
Volume	145
Issue number	C
DOIs	https://doi.org/10.1016/s0923-2516(07)80022-7
State	Published - 1994
Externally published	Yes

Keywords

HIV
Immunocytochemistry
In situ hybridization
Interleukin
Macrophage
Monocyte
TNF

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/s0923-2516(07)80022-7

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title = "Cytokine expression of HIV-infected monocytes/macrophages at the single-cell level",

abstract = "Monocytes of healthy donors were infected with HIV1 in vitro: 14-21 days after infection 50-70 % of the cells produced p24 HIV1 antigen as detected with anti-p24 immunostaining; infected cultures showed enhanced secretion of interleukin-6 (IL6), interleukin-8 (IL8) and tumour necrosis factor alpha (TNF-alpha). The expression of cytokines on the single-cell level was further analysed by in situ hybridization using non-radioactive digoxigenin for detection. HIV1 (p24+)-producing cells were compared with non-HIV (p24-) -producing cells. All morphological subtypes of macrophages showed HIV production; no difference in cytokine expression was observed. Immunocytochemistry of HIV-infected and uninfected cultures also showed no difference in the pattern of IL1-beta, IL6, IL8 and TNF-alpha protein expression in the cells.",

keywords = "HIV, Immunocytochemistry, In situ hybridization, Interleukin, Macrophage, Monocyte, TNF",

author = "W. Glienke and R. Esser and {von Briesen}, H. and K. Schuster and S. M{\"u}ller and R. Unger and R. Andreesen and Stutte, {H. J.} and H. R{\"u}bsamen-Waigmann",

year = "1994",

doi = "10.1016/s0923-2516(07)80022-7",

language = "אנגלית",

volume = "145",

pages = "193--197",

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T1 - Cytokine expression of HIV-infected monocytes/macrophages at the single-cell level

AU - Glienke, W.

AU - Esser, R.

AU - von Briesen, H.

AU - Schuster, K.

AU - Müller, S.

AU - Unger, R.

AU - Andreesen, R.

AU - Stutte, H. J.

AU - Rübsamen-Waigmann, H.

PY - 1994

Y1 - 1994

N2 - Monocytes of healthy donors were infected with HIV1 in vitro: 14-21 days after infection 50-70 % of the cells produced p24 HIV1 antigen as detected with anti-p24 immunostaining; infected cultures showed enhanced secretion of interleukin-6 (IL6), interleukin-8 (IL8) and tumour necrosis factor alpha (TNF-alpha). The expression of cytokines on the single-cell level was further analysed by in situ hybridization using non-radioactive digoxigenin for detection. HIV1 (p24+)-producing cells were compared with non-HIV (p24-) -producing cells. All morphological subtypes of macrophages showed HIV production; no difference in cytokine expression was observed. Immunocytochemistry of HIV-infected and uninfected cultures also showed no difference in the pattern of IL1-beta, IL6, IL8 and TNF-alpha protein expression in the cells.

AB - Monocytes of healthy donors were infected with HIV1 in vitro: 14-21 days after infection 50-70 % of the cells produced p24 HIV1 antigen as detected with anti-p24 immunostaining; infected cultures showed enhanced secretion of interleukin-6 (IL6), interleukin-8 (IL8) and tumour necrosis factor alpha (TNF-alpha). The expression of cytokines on the single-cell level was further analysed by in situ hybridization using non-radioactive digoxigenin for detection. HIV1 (p24+)-producing cells were compared with non-HIV (p24-) -producing cells. All morphological subtypes of macrophages showed HIV production; no difference in cytokine expression was observed. Immunocytochemistry of HIV-infected and uninfected cultures also showed no difference in the pattern of IL1-beta, IL6, IL8 and TNF-alpha protein expression in the cells.

KW - HIV

KW - Immunocytochemistry

KW - In situ hybridization

KW - Interleukin

KW - Macrophage

KW - Monocyte

KW - TNF

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AN - SCOPUS:0027999216

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VL - 145

SP - 193

EP - 197

JO - Research in Virology

JF - Research in Virology

IS - C

ER -

Cytokine expression of HIV-infected monocytes/macrophages at the single-cell level

Abstract

Keywords

UN SDGs

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