Cyclopropenium nanoparticles and gene transfection in cells

Noam Y. Steinman, Luis M. Campos, Yakai Feng, Abraham J. Domb, Hossein Hosseinkhani

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Non-viral vectors for the transfection of genetic material are at the frontier of medical science. In this article, we introduce for the first time, cyclopropenium-containing nanoparticles as a cationic carrier for gene transfection, as an alternative to the common quaternary ammonium transfection agents. Cyclopropenium-based cationic nanoparticles were prepared by crosslinking poly(ethylene imine) (PEI) with tetrachlorocyclopropene. These nanoparticles were electrostatically complexed with plasmid DNA into nanoparticles (~50 nm). Their cellular uptake into F929 mouse fibroblast cells, and their eventual expression in vitro have been described. Transfection is enhanced relative to PEI with minimal toxicity. These cyclopropenium nanoparticles possess efficient gene transfection capabilities with minimal cytotoxicity, which makes them novel and promising candidates for gene therapy.

Original languageEnglish
Article number768
Pages (from-to)1-12
Number of pages12
JournalPharmaceutics
Volume12
Issue number8
DOIs
StatePublished - 13 Aug 2020
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

  • Cationic nanoparticles
  • Cyclopropenium
  • Gene transfection
  • Poly(ethylene imine)

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