Cusp-artifacts in high order superresolution optical fluctuation imaging

Xiyu Yi, Shimon Weiss

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Superresolution optical fluctuation imaging (SOFI) is a simple and affordable superresolution imaging technique, and attracted a growing community over the past decade. However, the theoretical resolution enhancement of high order SOFI is still not fulfilled. In this study, we identify “cusp artifacts” in high order SOFI images, and show that the high-order cumulants, odd-order moments and balanced-cumulants (bSOFI) are highly vulnerable to cusp artifacts. Our study provides guidelines for developing and screening for fluorescence probes, and improving data acquisition for SOFI. The new insight is important to inspire positive utilization of the cusp artifacts.

Original languageEnglish
Pages (from-to)554-570
Number of pages17
JournalBiomedical Optics Express
Issue number2
StatePublished - 1 Feb 2020

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© 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.


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