TY - JOUR
T1 - Cultivable heterotrophic N2-fixing bacterial diversity in rice fields in the Yangtze River Plain
AU - Xie, Guang Hui
AU - Cai, Miao Ying
AU - Tao, Guang Can
AU - Steinberger, Yosef
PY - 2003/1/1
Y1 - 2003/1/1
N2 - Heterotrophic N2-fixing bacteria are a potentially important source of N2 fixation in rice fields due to the moist soil conditions. This study was conducted at eight sites along a geographic gradient of the Yangtze River Plain in central China. A nitrogen-free solid malate-sucrose medium was used to isolate heterotrophic N2-fixing bacteria. Numbers of the culturable N2-fixing bacteria expressed as CFU (colony forming units) ranged between 1.41±0.42×106 and 1.24±0.23×108 in the sampled paddy field sites along the plain. Thirty strains with high ARA (acetylene reduction activity) were isolated and purified; ARA of the strains varied from 0.9 to 537.8 nmol C2H4 culture-1 h-1, and amounts of 15N fixed ranged between 0.008 and 0.4866 mg·culture-1·day-1. According to morphological and biochemical characteristics, 14 strains were identified as the genus Bacillus, 2 as Burkholderia, 1 as Agrobacterium, 4 as Pseudomonas, 2 as Derxia, 1 as Alcaligenes, 1 as Aeromonas, 2 as Citrobacter, and 3 strains belonged to the corynebacterform group.
AB - Heterotrophic N2-fixing bacteria are a potentially important source of N2 fixation in rice fields due to the moist soil conditions. This study was conducted at eight sites along a geographic gradient of the Yangtze River Plain in central China. A nitrogen-free solid malate-sucrose medium was used to isolate heterotrophic N2-fixing bacteria. Numbers of the culturable N2-fixing bacteria expressed as CFU (colony forming units) ranged between 1.41±0.42×106 and 1.24±0.23×108 in the sampled paddy field sites along the plain. Thirty strains with high ARA (acetylene reduction activity) were isolated and purified; ARA of the strains varied from 0.9 to 537.8 nmol C2H4 culture-1 h-1, and amounts of 15N fixed ranged between 0.008 and 0.4866 mg·culture-1·day-1. According to morphological and biochemical characteristics, 14 strains were identified as the genus Bacillus, 2 as Burkholderia, 1 as Agrobacterium, 4 as Pseudomonas, 2 as Derxia, 1 as Alcaligenes, 1 as Aeromonas, 2 as Citrobacter, and 3 strains belonged to the corynebacterform group.
KW - Acetylene reduction activity
KW - N
KW - N-fixing bacteria
KW - Paddy field
UR - http://www.scopus.com/inward/record.url?scp=0037284299&partnerID=8YFLogxK
U2 - 10.1007/s00374-002-0565-2
DO - 10.1007/s00374-002-0565-2
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AN - SCOPUS:0037284299
SN - 0178-2762
VL - 37
SP - 29
EP - 38
JO - Biology and Fertility of Soils
JF - Biology and Fertility of Soils
IS - 1
ER -