Cryobanking of primary somatic cells of elite farm animals - A pilot study in domesticated water buffalo (Bubalus bubalis)

Seema Dua, Papori Sharma, Monika Saini, Nidhi Rawat, Rasika Rajendran, Sonu Bansal, Abubakar Muhammad Wakil, Madhuri Beniwal, Atul Parashar, Kamlesh Kumari Bajwa, Naresh L. Selokar, Rajesh Kumar, Dharmendra Kumar, Prem Singh Yadav

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

Buffalo is an important farm animal species in South and South-east Asian countries. Cryopreservation allows long-term storage of somatic cells, which can be made available to research communities. This study aimed to 1) establish and cryopreserve somatic cells from elite buffaloes, and 2) share stored somatic cells and their associated data with researchers. To achieve these targets, somatic cells were established successfully from tail-skin biopsies of 17 buffaloes. The informative data such as buffalo details (breed, date of birth, sex, and age at the time of tissue biopsy collection, and production traits), the number of cryovials stored, and freezing dates were recorded in an electronic file and a printed inventory record. The established somatic cells were flat, spindle-shaped morphology, and expressed vimentin (a fibroblast-like cell type marker) and the negative expression of cytokeratin-18 (an epithelial cell type marker). Altogether, we cryopreserved 970 cryovials (0.1 million cells per vial) from two buffalo breeds, namely Murrah and Nili-Ravi (at least 45 cryovials per animal), for cryobanking. Somatic cell nuclear transfer (SCNT) experiments demonstrated the utility of cryopreserved cells to produce cloned buffaloes. Importantly, these cryopreserved somatic cells are made available to scientific communities. This study encourages the cryopreservation of somatic cells of elite farm animals for their utilization in cell-based research.

Original languageEnglish
Pages (from-to)139-145
Number of pages7
JournalCryobiology
Volume98
DOIs
StatePublished - Feb 2021
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2020 Elsevier Inc.

Funding

The present study was financed by the Indian Council of Agricultural Research, Delhi, India (NASF/GTR-7004/2018-19), and Science and Engineering Research Board, Delhi, India (YSS/2014/000278). We are thankful to Director, ICAR-Central Institute for Research on Buffaloes (CIRB) for providing necessary facilities to initiate somatic cell cryobanking in buffalo.

FundersFunder number
Indian Council of Agricultural Research, Delhi, IndiaNASF/GTR-7004/2018-19
Science and Engineering Research Board, Delhi, IndiaYSS/2014/000278

    Keywords

    • Buffalo
    • Farm animals
    • Fibroblast cells
    • Somatic cell cryobank
    • Somatic cell nuclear transfer

    Fingerprint

    Dive into the research topics of 'Cryobanking of primary somatic cells of elite farm animals - A pilot study in domesticated water buffalo (Bubalus bubalis)'. Together they form a unique fingerprint.

    Cite this