Abstract
Epstein-Barr virus (EBV) is associated with a variety of tumors and nonmalignant conditions. Latent EBV genomes in cells, including tumor cells, are often CpG methylated, whereas virion DNA is not CpG methylated. We demonstrate that methyl CpG binding magnetic beads can be used to fractionate among sources of EBV DNA (DNA extracted from laboratory-purified virions vs DNA extracted from latently infected cell lines). We then applied the technique to plasma specimens and showed that this technique can distinguish EBV DNA from patients with EBV-associated tumors (nasopharyngeal carcinoma, Hodgkin lymphoma) and viral DNA from patients without EBV-associated tumors, including immunocompromised patients and patients with EBV(2) Hodgkin lymphoma.
| Original language | English |
|---|---|
| Pages (from-to) | 1624-1627 |
| Number of pages | 4 |
| Journal | Blood advances |
| Volume | 4 |
| Issue number | 8 |
| DOIs | |
| State | Published - 28 Apr 2020 |
Bibliographical note
Publisher Copyright:© 2020 American Society of Hematology. All rights reserved.
Funding
This work was supported by grants from the National Institutes of Health, National Cancer Institute (P50CA96888, U01CA121947, P30CA006973, and P01CA113239) (R.F.A.), a grant from the Singapore National Research Foundation and the Ministry of Education under the Research Center of Excellence Program (W.-s.H. and R.F.A.), and grants from the Israel Cancer Association (20150095 and 20161143) funded by Walter Bela foundation, and Research Career Development Award from the Israel Cancer Research Fund (01282) (M.S.). The authors are grateful for the support of the Elias, Genevieve and Georgianna Atol Charitable Trust to the Daniella Lee Casper Laboratory in Viral Oncology.
| Funders | Funder number |
|---|---|
| Walter Bela foundation | |
| National Institutes of Health | |
| National Cancer Institute | P30CA006973, P01CA113239, U01CA121947, P50CA96888 |
| Israel Cancer Research Fund | 01282 |
| National Research Foundation Singapore | |
| Ministry of Education | |
| Israel Cancer Association | 20161143, 20150095 |