CpG methylation in cell-free Epstein-Barr virus DNA in patients with EBV-hodgkin lymphoma

Meir Shamay; Jennifer A. Kanakry; John S.W. Low; Netanel A. Horowitz; Guy Journo; Anuj Ahuja; Yonatan Eran; Elinor Barzilai; Eldad J. Dann; Jennifer Stone; Wan Lu Woo; Wen Son Hsieh; Rena R. Xian; Richard F. Ambinder

doi:10.1182/bloodadvances.2020001511

CpG methylation in cell-free Epstein-Barr virus DNA in patients with EBV-hodgkin lymphoma

Meir Shamay, Jennifer A. Kanakry, John S.W. Low, Netanel A. Horowitz, Guy Journo, Anuj Ahuja, Yonatan Eran, Elinor Barzilai, Eldad J. Dann, Jennifer Stone, Wan Lu Woo, Wen Son Hsieh, Rena R. Xian, Richard F. Ambinder

Bar-Ilan University - Azrieli Faculty of Medicine

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Epstein-Barr virus (EBV) is associated with a variety of tumors and nonmalignant conditions. Latent EBV genomes in cells, including tumor cells, are often CpG methylated, whereas virion DNA is not CpG methylated. We demonstrate that methyl CpG binding magnetic beads can be used to fractionate among sources of EBV DNA (DNA extracted from laboratory-purified virions vs DNA extracted from latently infected cell lines). We then applied the technique to plasma specimens and showed that this technique can distinguish EBV DNA from patients with EBV-associated tumors (nasopharyngeal carcinoma, Hodgkin lymphoma) and viral DNA from patients without EBV-associated tumors, including immunocompromised patients and patients with EBV(2) Hodgkin lymphoma.

Original language	English
Pages (from-to)	1624-1627
Number of pages	4
Journal	Blood advances
Volume	4
Issue number	8
DOIs	https://doi.org/10.1182/bloodadvances.2020001511
State	Published - 28 Apr 2020

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© 2020 American Society of Hematology. All rights reserved.

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title = "CpG methylation in cell-free Epstein-Barr virus DNA in patients with EBV-hodgkin lymphoma",

abstract = "Epstein-Barr virus (EBV) is associated with a variety of tumors and nonmalignant conditions. Latent EBV genomes in cells, including tumor cells, are often CpG methylated, whereas virion DNA is not CpG methylated. We demonstrate that methyl CpG binding magnetic beads can be used to fractionate among sources of EBV DNA (DNA extracted from laboratory-purified virions vs DNA extracted from latently infected cell lines). We then applied the technique to plasma specimens and showed that this technique can distinguish EBV DNA from patients with EBV-associated tumors (nasopharyngeal carcinoma, Hodgkin lymphoma) and viral DNA from patients without EBV-associated tumors, including immunocompromised patients and patients with EBV(2) Hodgkin lymphoma.",

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AU - Shamay, Meir

AU - Kanakry, Jennifer A.

AU - Low, John S.W.

AU - Horowitz, Netanel A.

AU - Journo, Guy

AU - Ahuja, Anuj

AU - Eran, Yonatan

AU - Barzilai, Elinor

AU - Dann, Eldad J.

AU - Stone, Jennifer

AU - Woo, Wan Lu

AU - Hsieh, Wen Son

AU - Xian, Rena R.

AU - Ambinder, Richard F.

PY - 2020/4/28

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AB - Epstein-Barr virus (EBV) is associated with a variety of tumors and nonmalignant conditions. Latent EBV genomes in cells, including tumor cells, are often CpG methylated, whereas virion DNA is not CpG methylated. We demonstrate that methyl CpG binding magnetic beads can be used to fractionate among sources of EBV DNA (DNA extracted from laboratory-purified virions vs DNA extracted from latently infected cell lines). We then applied the technique to plasma specimens and showed that this technique can distinguish EBV DNA from patients with EBV-associated tumors (nasopharyngeal carcinoma, Hodgkin lymphoma) and viral DNA from patients without EBV-associated tumors, including immunocompromised patients and patients with EBV(2) Hodgkin lymphoma.

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CpG methylation in cell-free Epstein-Barr virus DNA in patients with EBV-hodgkin lymphoma

Abstract

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