CpG methylation as a tool to characterize cell-free Kaposi sarcoma herpesvirus DNA

Meir Shamay, Nicholas Hand, M. Victor Lemas, Henry B. Koon, Susan E. Krown, John Wrangle, Prashant Desai, Juan Carlos Ramos, Richard F. Ambinder

Research output: Contribution to journalArticlepeer-review

16 Scopus citations


We studied the presence of Kaposi sarcoma herpesvirus sequences in cell-free DNA (cfDNA) isolated from the blood of patients with AIDS-related Kaposi sarcoma (KS) and primary effusion lymphoma (PEL). The use of paramagnetic beads linked to methyl-CpG binding domain protein allowed separation of virion and cell-derived DNA. Only virion DNA was detected in the blood of KS patients, whereas cell-derived DNA was detected in a patient with AIDS-related PEL. The difference in the origins of cfDNA in these settings may in part reflect very different proliferative indices in KS and PEL tumor tissue.

Original languageEnglish
Pages (from-to)1095-1099
Number of pages5
JournalJournal of Infectious Diseases
Issue number7
StatePublished - 1 Apr 2012
Externally publishedYes

Bibliographical note

Funding Information:
Financial support. This work was supported by grants from the National Institutes of Health (P50CA96888, U01CA121947, P30CA006973, P01CA113239). Potential conflicts of interest. All authors: No reported conflicts. All authors have submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest. Conflicts that the editors consider relevant to the content of the manuscript have been disclosed.


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