TY - JOUR
T1 - Courtship Conditioning/Suppression Assays in Drosophila
AU - von Philipsborn, Anne C.
AU - Shohat-Ophir, Galit
AU - Rezaval, Carolina
N1 - Publisher Copyright:
© 2023 Cold Spring Harbor Laboratory Press.
PY - 2023/7
Y1 - 2023/7
N2 - Naive males court both virgin and mated females but learn through experience to discriminate between them, thus minimizing futile investments in nonreceptive female flies. In the laboratory, we can exploit the innate courtship enthusiasm of males and manipulate their behavior by placing them with a nonreceptive female (immature virgin females, decapitated mature virgin females, or mature mated females), termed as the courtship suppression/conditioning assay. Early studies showed that male flies that experience failure to mate upon interaction with nonreceptive previously mated females show decreased motivation to court (courtship suppression). Courtship suppression is an important experimental paradigm for studying genes and neuronal circuits that mediate short- and long-term memory. The anti-aphrodisiac male-specific pheromone 11-cis-vaccenyl-acetate plays a key role in this conditioned response, as male flies learn to associate its presence on mated females with the failure to mate.
AB - Naive males court both virgin and mated females but learn through experience to discriminate between them, thus minimizing futile investments in nonreceptive female flies. In the laboratory, we can exploit the innate courtship enthusiasm of males and manipulate their behavior by placing them with a nonreceptive female (immature virgin females, decapitated mature virgin females, or mature mated females), termed as the courtship suppression/conditioning assay. Early studies showed that male flies that experience failure to mate upon interaction with nonreceptive previously mated females show decreased motivation to court (courtship suppression). Courtship suppression is an important experimental paradigm for studying genes and neuronal circuits that mediate short- and long-term memory. The anti-aphrodisiac male-specific pheromone 11-cis-vaccenyl-acetate plays a key role in this conditioned response, as male flies learn to associate its presence on mated females with the failure to mate.
UR - http://www.scopus.com/inward/record.url?scp=85164245162&partnerID=8YFLogxK
U2 - 10.1101/pdb.prot108106
DO - 10.1101/pdb.prot108106
M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???
C2 - 36781210
AN - SCOPUS:85164245162
SN - 1940-3402
VL - 2023
SP - 459
EP - 466
JO - Cold Spring Harbor Protocols
JF - Cold Spring Harbor Protocols
IS - 7
ER -