Coupled pre-mRNA and mRNA dynamics unveil operational strategies underlying transcriptional responses to stimuli

Amit Zeisel; Wolfgang J. Köstler; Natali Molotski; Jonathan M. Tsai; Rita Krauthgamer; Jasmine Jacob-Hirsch; Gideon Rechavi; Yoav Soen; Steffen Jung; Yosef Yarden; Eytan Domany

doi:10.1038/msb.2011.62

Coupled pre-mRNA and mRNA dynamics unveil operational strategies underlying transcriptional responses to stimuli

Amit Zeisel, Wolfgang J. Köstler, Natali Molotski, Jonathan M. Tsai, Rita Krauthgamer, Jasmine Jacob-Hirsch, Gideon Rechavi, Yoav Soen, Steffen Jung, Yosef Yarden, Eytan Domany

Research output: Contribution to journal › Review article › peer-review

107 Scopus citations

Abstract

Transcriptional responses to extracellular stimuli involve tuning the rates of transcript production and degradation. Here, we show that the time-dependent profiles of these rates can be inferred from simultaneous measurements of precursor mRNA (pre-mRNA) and mature mRNA profiles. Transcriptome-wide measurements demonstrate that genes with similar mRNA profiles often exhibit marked differences in the amplitude and onset of their production rate. The latter is characterized by a large dynamic range, with a group of genes exhibiting an unexpectedly strong transient production overshoot, thereby accelerating their induction and, when combined with time-dependent degradation, shaping transient responses with precise timing and amplitude.

Original language	English
Article number	529
Journal	Molecular Systems Biology
Volume	7
DOIs	https://doi.org/10.1038/msb.2011.62
State	Published - 13 Sep 2011
Externally published	Yes

Funding

Funders	Funder number
National Cancer Institute	R37CA072981

Keywords

half-life
operational strategy
pre-mRNA
transcriptional response

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10.1038/msb.2011.62

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abstract = "Transcriptional responses to extracellular stimuli involve tuning the rates of transcript production and degradation. Here, we show that the time-dependent profiles of these rates can be inferred from simultaneous measurements of precursor mRNA (pre-mRNA) and mature mRNA profiles. Transcriptome-wide measurements demonstrate that genes with similar mRNA profiles often exhibit marked differences in the amplitude and onset of their production rate. The latter is characterized by a large dynamic range, with a group of genes exhibiting an unexpectedly strong transient production overshoot, thereby accelerating their induction and, when combined with time-dependent degradation, shaping transient responses with precise timing and amplitude.",

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AU - Zeisel, Amit

AU - Köstler, Wolfgang J.

AU - Molotski, Natali

AU - Tsai, Jonathan M.

AU - Krauthgamer, Rita

AU - Jacob-Hirsch, Jasmine

AU - Rechavi, Gideon

AU - Soen, Yoav

AU - Jung, Steffen

AU - Yarden, Yosef

AU - Domany, Eytan

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AB - Transcriptional responses to extracellular stimuli involve tuning the rates of transcript production and degradation. Here, we show that the time-dependent profiles of these rates can be inferred from simultaneous measurements of precursor mRNA (pre-mRNA) and mature mRNA profiles. Transcriptome-wide measurements demonstrate that genes with similar mRNA profiles often exhibit marked differences in the amplitude and onset of their production rate. The latter is characterized by a large dynamic range, with a group of genes exhibiting an unexpectedly strong transient production overshoot, thereby accelerating their induction and, when combined with time-dependent degradation, shaping transient responses with precise timing and amplitude.

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KW - pre-mRNA

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Coupled pre-mRNA and mRNA dynamics unveil operational strategies underlying transcriptional responses to stimuli

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Keywords

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