Correlation of magnetic AC field on cardiac myocyte Ca2+ transients at different magnetic DC levels

Dror Fixler, Smadar Yitzhaki, Alexander Axelrod, Tova Zinman, Asher Shainberg

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

The purpose of this study was to determine the effect of extremely low frequency and weak magnetic fields (WMF) on cardiac myocyte Ca2+ transients, and to explore the involvement of potassium channels under the WMF effect. In addition, we aimed to find a physical explanation for the effect of WMF on cardiac myocyte Ca2+ transients. Indo-1 loaded cells, which were exposed to a WMF at 16Hz and 40nT, demonstrated a 75±4% reduction in cytosolic Ca2+ transients versus control. Treatment with the KATP channel blocker, glibenclamide, followed by WMF at 16Hz exposure, blocked the reduction in cytosolic calcium transients while treatment with pinacidil, a KATP channel opener, or chromanol 293B, a selective potassium channel blocker of the delayed rectifier K+ channels, did not inhibit the effect. Based on these finding and the ion cyclotron resonance frequency theory, we further investigated the effect of WMF by changing the direct current (DC) magnetic field (B0). When operating different DC magnetic fields we showed that the WMF value changed correspondingly: for B0=44.5μT, the effect was observed at 17.05Hz; for B0=46.5μT, the effect was observed at 18.15Hz; and for B0=49μT the effect was observed at 19.1Hz. We can conclude that the effect of WMF on Ca2+ transients depends on the DC magnetic field level.

Original languageEnglish
Pages (from-to)634-640
Number of pages7
JournalBioelectromagnetics
Volume33
Issue number8
DOIs
StatePublished - Dec 2012

Keywords

  • Alternating current
  • Direct current
  • Extremely low frequencies
  • Ion cyclotron resonance
  • Magnetic field
  • Potassium channels
  • Weak magnetic field

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