TY - JOUR
T1 - Correlation of cyclooxygenase-2 (COX-2) and aromatase expression in human endometrial cancer
T2 - Tissue microarray analysis
AU - Fowler, Jeffrey M.
AU - Ramirez, Nilsa
AU - Cohn, David E.
AU - Kelbick, Nicole
AU - Pavelka, James
AU - Ben-Shachar, Inbar
AU - Morrison, Carl
PY - 2005/4
Y1 - 2005/4
N2 - Objective: The objective of this study was to compare the prevalence of cyclooxygenase-2 (COX-2), aromatase, and hormone receptor immunohistochemical (IHC) expression to well defined clinical-pathologic prognostic factors in a large group of surgically staged endometrial cancer patients. Study design: A tissue microarray (TMA) was constructed from 336 separate specimens of endometrial cancer. IHC was perfomed for estrogen (ER) and progesterone (PR) receptor, COX-2, COX-1, and aromatase. Results: The majority of tumors expressed COX-2 (59%) and aromatase (65%). COX-2 staining significantly correlated with aromatase expression (P < .014) but did not correlate with ER and PR. COX-2 expression was correlated with worsening histologic grade (P < .026) and approached statistical significance for deep myometrial invasion (P < .055). After applying multivariate analysis, no single IHC or combination of IHCs correlated with intrauterine poor prognostic factors or advanced stage. Only myometrial invasion >50% (OR 6.98, P < .001) and nonendometrioid histology (OR 4.933, P < .001) were predictive of advanced stage after multivariate analysis. Conclusion: COX-2 and aromatase are expressed in the majority of endometrial cancer patients. COX-2 expression was not associated with the great majority of surgical-pathologic prognostic factors. COX-2 expression did significantly correlate with aromatase expression, suggesting that intratumoral production of estrogen in endometrial cancer may be an important mechanism in tumorigenesis.
AB - Objective: The objective of this study was to compare the prevalence of cyclooxygenase-2 (COX-2), aromatase, and hormone receptor immunohistochemical (IHC) expression to well defined clinical-pathologic prognostic factors in a large group of surgically staged endometrial cancer patients. Study design: A tissue microarray (TMA) was constructed from 336 separate specimens of endometrial cancer. IHC was perfomed for estrogen (ER) and progesterone (PR) receptor, COX-2, COX-1, and aromatase. Results: The majority of tumors expressed COX-2 (59%) and aromatase (65%). COX-2 staining significantly correlated with aromatase expression (P < .014) but did not correlate with ER and PR. COX-2 expression was correlated with worsening histologic grade (P < .026) and approached statistical significance for deep myometrial invasion (P < .055). After applying multivariate analysis, no single IHC or combination of IHCs correlated with intrauterine poor prognostic factors or advanced stage. Only myometrial invasion >50% (OR 6.98, P < .001) and nonendometrioid histology (OR 4.933, P < .001) were predictive of advanced stage after multivariate analysis. Conclusion: COX-2 and aromatase are expressed in the majority of endometrial cancer patients. COX-2 expression was not associated with the great majority of surgical-pathologic prognostic factors. COX-2 expression did significantly correlate with aromatase expression, suggesting that intratumoral production of estrogen in endometrial cancer may be an important mechanism in tumorigenesis.
KW - Aromatase
KW - Cyclooxygenase-2
KW - Endometrial carcinoma
KW - Tissue microarray
UR - http://www.scopus.com/inward/record.url?scp=16844364010&partnerID=8YFLogxK
U2 - 10.1016/j.ajog.2005.01.009
DO - 10.1016/j.ajog.2005.01.009
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C2 - 15846217
AN - SCOPUS:16844364010
SN - 0002-9378
VL - 192
SP - 1262
EP - 1271
JO - American Journal of Obstetrics and Gynecology
JF - American Journal of Obstetrics and Gynecology
IS - 4
ER -
