COP9 signalosome subunit 7 from Arabidopsis interacts with and regulates the small subunit of ribonucleotide reductase (RNR2)

Yair Halimi; Moshe Dessau; Shaul Pollak; Tslil Ast; Tamir Erez; Nurit Livnat-Levanon; Baruch Karniol; Joel A. Hirsch; Daniel A. Chamovitz

doi:10.1007/s11103-011-9795-8

COP9 signalosome subunit 7 from Arabidopsis interacts with and regulates the small subunit of ribonucleotide reductase (RNR2)

Yair Halimi, Moshe Dessau, Shaul Pollak, Tslil Ast, Tamir Erez, Nurit Livnat-Levanon, Baruch Karniol, Joel A. Hirsch, Daniel A. Chamovitz

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

The COP9 Signalosome protein complex (CSN) is a pleiotropic regulator of plant development and contains eight-subunits. Six of these subunits contain the PCI motif which mediates specific protein interactions necessary for the integrity of the complex. COP9 complex subunit 7 (CSN7) contains an N-terminal PCI motif followed by a C-terminal extension which is also necessary for CSN function. A yeast-interaction trap assay identified the small subunit of ribonucelotide reductase (RNR2) from Arabidopsis as interacting with the C-terminal section of CSN7. This interaction was confirmed in planta by both bimolecular fluorescence complementation and immuoprecipitation assays with endogenous proteins. The subcellular localization of RNR2 was primarily nuclear in meristematic regions, and cytoplasmic in adult cells. RNR2 was constitutively nuclear in csn7 mutant seedlings, and was also primarily nuclear in wild type seedlings following exposure to UV-C. These two results correlate with constitutive expression of several DNA-damage response genes in csn7 mutants, and to increased tolerance of csn7 seedlings to UV-C treatment. We propose that the CSN is a negative regulator of RNR activity in Arabidopsis.

Original language	English
Pages (from-to)	77-89
Number of pages	13
Journal	Plant Molecular Biology
Volume	77
Issue number	1
DOIs	https://doi.org/10.1007/s11103-011-9795-8
State	Published - Sep 2011
Externally published	Yes

Bibliographical note

Funding Information:
Acknowledgments We thank Professor Nir Ohad with assistance and use of the confocal microscope, and Professor Amram Eshel for assistance with PAM. YH is the recipient of an Eshkol Foundation predoctoral fellowship. MD was supported in part by a matching Tel Aviv University Rector doctoral fellowship. This work was supported by a grant from Israel Science Foundation (783/05) to DAC and JAH, and from the Israel—US Binational Agricultural Research and Development Fund (IS-2775-96R) to DAC.

Funding

Acknowledgments We thank Professor Nir Ohad with assistance and use of the confocal microscope, and Professor Amram Eshel for assistance with PAM. YH is the recipient of an Eshkol Foundation predoctoral fellowship. MD was supported in part by a matching Tel Aviv University Rector doctoral fellowship. This work was supported by a grant from Israel Science Foundation (783/05) to DAC and JAH, and from the Israel—US Binational Agricultural Research and Development Fund (IS-2775-96R) to DAC.

Funders	Funder number
Eshkol Foundation
United States - Israel Binational Agricultural Research and Development Fund	IS-2775-96R
Israel Science Foundation	783/05
Tel Aviv University

Keywords

Arabidopsis
Cop9 signalosome
DNA damage
PCI
Ribonucleotide reductase

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10.1007/s11103-011-9795-8

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title = "COP9 signalosome subunit 7 from Arabidopsis interacts with and regulates the small subunit of ribonucleotide reductase (RNR2)",

abstract = "The COP9 Signalosome protein complex (CSN) is a pleiotropic regulator of plant development and contains eight-subunits. Six of these subunits contain the PCI motif which mediates specific protein interactions necessary for the integrity of the complex. COP9 complex subunit 7 (CSN7) contains an N-terminal PCI motif followed by a C-terminal extension which is also necessary for CSN function. A yeast-interaction trap assay identified the small subunit of ribonucelotide reductase (RNR2) from Arabidopsis as interacting with the C-terminal section of CSN7. This interaction was confirmed in planta by both bimolecular fluorescence complementation and immuoprecipitation assays with endogenous proteins. The subcellular localization of RNR2 was primarily nuclear in meristematic regions, and cytoplasmic in adult cells. RNR2 was constitutively nuclear in csn7 mutant seedlings, and was also primarily nuclear in wild type seedlings following exposure to UV-C. These two results correlate with constitutive expression of several DNA-damage response genes in csn7 mutants, and to increased tolerance of csn7 seedlings to UV-C treatment. We propose that the CSN is a negative regulator of RNR activity in Arabidopsis.",

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author = "Yair Halimi and Moshe Dessau and Shaul Pollak and Tslil Ast and Tamir Erez and Nurit Livnat-Levanon and Baruch Karniol and Hirsch, {Joel A.} and Chamovitz, {Daniel A.}",

note = "Funding Information: Acknowledgments We thank Professor Nir Ohad with assistance and use of the confocal microscope, and Professor Amram Eshel for assistance with PAM. YH is the recipient of an Eshkol Foundation predoctoral fellowship. MD was supported in part by a matching Tel Aviv University Rector doctoral fellowship. This work was supported by a grant from Israel Science Foundation (783/05) to DAC and JAH, and from the Israel—US Binational Agricultural Research and Development Fund (IS-2775-96R) to DAC.",

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AU - Erez, Tamir

AU - Livnat-Levanon, Nurit

AU - Karniol, Baruch

AU - Hirsch, Joel A.

AU - Chamovitz, Daniel A.

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N2 - The COP9 Signalosome protein complex (CSN) is a pleiotropic regulator of plant development and contains eight-subunits. Six of these subunits contain the PCI motif which mediates specific protein interactions necessary for the integrity of the complex. COP9 complex subunit 7 (CSN7) contains an N-terminal PCI motif followed by a C-terminal extension which is also necessary for CSN function. A yeast-interaction trap assay identified the small subunit of ribonucelotide reductase (RNR2) from Arabidopsis as interacting with the C-terminal section of CSN7. This interaction was confirmed in planta by both bimolecular fluorescence complementation and immuoprecipitation assays with endogenous proteins. The subcellular localization of RNR2 was primarily nuclear in meristematic regions, and cytoplasmic in adult cells. RNR2 was constitutively nuclear in csn7 mutant seedlings, and was also primarily nuclear in wild type seedlings following exposure to UV-C. These two results correlate with constitutive expression of several DNA-damage response genes in csn7 mutants, and to increased tolerance of csn7 seedlings to UV-C treatment. We propose that the CSN is a negative regulator of RNR activity in Arabidopsis.

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COP9 signalosome subunit 7 from Arabidopsis interacts with and regulates the small subunit of ribonucleotide reductase (RNR2)

Abstract

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Keywords

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