Cooperative interactions at the SLP‐76 complex are critical for actin polymerization

Mira Barda‐Saad; N Shirasu; M.H Pauker; N Hassan; O Perl; A Balbo; H Yamaguchi; J.C Houtman; E Appella; P Schuck; L.E Samelson

Cooperative interactions at the SLP‐76 complex are critical for actin polymerization

Mira Barda‐Saad, N Shirasu, M.H Pauker, N Hassan, O Perl, A Balbo, H Yamaguchi, J.C Houtman, E Appella, P Schuck, L.E Samelson

The Mina and Everard Goodman Faculty of Life Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

T‐cell antigen receptor (TCR) engagement induces formation of multi‐protein signalling complexes essential for regulating T‐cell functions. Generation of a complex of SLP‐76, Nck and VAV1 is crucial for regulation of the actin machinery. We define the composition, stoichiometry and specificity of interactions in the SLP‐76, Nck and VAV1 complex. Our data reveal that this complex can contain one SLP‐76 molecule, two Nck and two VAV1 molecules. A direct interaction between Nck and VAV1 is mediated by binding between the C‐terminal SH3 domain of Nck and the VAV1 N‐terminal SH3 domain. Disruption of the VAV1:Nck interaction deleteriously affected actin polymerization. These novel findings shed new light on the mechanism of actin polymerization after T‐cell activation.

Original language	American English
Pages (from-to)	2315-2328
Journal	The EMBO Journal
Volume	29
Issue number	14
State	Published - 2010

Cite this

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title = "Cooperative interactions at the SLP‐76 complex are critical for actin polymerization",

abstract = "T‐cell antigen receptor (TCR) engagement induces formation of multi‐protein signalling complexes essential for regulating T‐cell functions. Generation of a complex of SLP‐76, Nck and VAV1 is crucial for regulation of the actin machinery. We define the composition, stoichiometry and specificity of interactions in the SLP‐76, Nck and VAV1 complex. Our data reveal that this complex can contain one SLP‐76 molecule, two Nck and two VAV1 molecules. A direct interaction between Nck and VAV1 is mediated by binding between the C‐terminal SH3 domain of Nck and the VAV1 N‐terminal SH3 domain. Disruption of the VAV1:Nck interaction deleteriously affected actin polymerization. These novel findings shed new light on the mechanism of actin polymerization after T‐cell activation.",

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AU - Barda‐Saad, Mira

AU - Shirasu, N

AU - Pauker, M.H

AU - Hassan, N

AU - Perl, O

AU - Balbo, A

AU - Yamaguchi, H

AU - Houtman, J.C

AU - Appella, E

AU - Schuck, P

AU - Samelson, L.E

PY - 2010

Y1 - 2010

N2 - T‐cell antigen receptor (TCR) engagement induces formation of multi‐protein signalling complexes essential for regulating T‐cell functions. Generation of a complex of SLP‐76, Nck and VAV1 is crucial for regulation of the actin machinery. We define the composition, stoichiometry and specificity of interactions in the SLP‐76, Nck and VAV1 complex. Our data reveal that this complex can contain one SLP‐76 molecule, two Nck and two VAV1 molecules. A direct interaction between Nck and VAV1 is mediated by binding between the C‐terminal SH3 domain of Nck and the VAV1 N‐terminal SH3 domain. Disruption of the VAV1:Nck interaction deleteriously affected actin polymerization. These novel findings shed new light on the mechanism of actin polymerization after T‐cell activation.

AB - T‐cell antigen receptor (TCR) engagement induces formation of multi‐protein signalling complexes essential for regulating T‐cell functions. Generation of a complex of SLP‐76, Nck and VAV1 is crucial for regulation of the actin machinery. We define the composition, stoichiometry and specificity of interactions in the SLP‐76, Nck and VAV1 complex. Our data reveal that this complex can contain one SLP‐76 molecule, two Nck and two VAV1 molecules. A direct interaction between Nck and VAV1 is mediated by binding between the C‐terminal SH3 domain of Nck and the VAV1 N‐terminal SH3 domain. Disruption of the VAV1:Nck interaction deleteriously affected actin polymerization. These novel findings shed new light on the mechanism of actin polymerization after T‐cell activation.

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M3 - Article

VL - 29

SP - 2315

EP - 2328

JO - The EMBO Journal

JF - The EMBO Journal

IS - 14

ER -

Cooperative interactions at the SLP‐76 complex are critical for actin polymerization

Abstract

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