@article{f1fc3cff6af7466394913699ae000b89,
title = "Cooperative interactions at the SLP-76 complex are critical for actin polymerization",
abstract = "T-cell antigen receptor (TCR) engagement induces formation of multi-protein signalling complexes essential for regulating T-cell functions. Generation of a complex of SLP-76, Nck and VAV1 is crucial for regulation of the actin machinery. We define the composition, stoichiometry and specificity of interactions in the SLP-76, Nck and VAV1 complex. Our data reveal that this complex can contain one SLP-76 molecule, two Nck and two VAV1 molecules. A direct interaction between Nck and VAV1 is mediated by binding between the C-terminal SH3 domain of Nck and the VAV1 N-terminal SH3 domain. Disruption of the VAV1:Nck interaction deleteriously affected actin polymerization. These novel findings shed new light on the mechanism of actin polymerization after T-cell activation.",
keywords = "SLP-76, actin polymerization, lymphocyte activation, signalling complexes",
author = "Mira Barda-Saad and Naoto Shirasu and Pauker, {Maor H.} and Nirit Hassan and Orly Perl and Andrea Balbo and Hiroshi Yamaguchi and Houtman, {Jon C.D.} and Ettore Appella and Peter Schuck and Samelson, {Lawrence E.}",
year = "2010",
month = jul,
day = "21",
doi = "10.1038/emboj.2010.133",
language = "אנגלית",
volume = "29",
pages = "2315--2328",
journal = "EMBO Journal",
issn = "0261-4189",
publisher = "Springer Science and Business Media Deutschland GmbH",
number = "14",
}