Control of Protein Activity and Gene Expression by Cyclofen-OH Uncaging

Weiting Zhang; Fatima Hamouri; Zhiping Feng; Isabelle Aujard; Bertrand Ducos; Shixin Ye; Shimon Weiss; Michel Volovitch; Sophie Vriz; Ludovic Jullien; David Bensimon

doi:10.1002/cbic.201700630

Control of Protein Activity and Gene Expression by Cyclofen-OH Uncaging

Weiting Zhang
, Fatima Hamouri
, Zhiping Feng
, Isabelle Aujard
, Bertrand Ducos
, Shixin Ye
, Shimon Weiss
, Michel Volovitch
, Sophie Vriz
, Ludovic Jullien
, David Bensimon

École normale supérieure
Université PSL
Stanford University
CNRS
Sorbonne Université
University of California at Los Angeles
Collège de France
Université Paris Cité

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

The use of light to control the expression of genes and the activity of proteins is a rapidly expanding field. Whereas many of these approaches use fusion between a light-activable protein and the protein of interest to control the activity of the latter, it is also possible to control the activity of a protein by uncaging a specific ligand. In that context, controlling the activation of a protein fused to the modified estrogen receptor (ERT) by uncaging its ligand cyclofen-OH has emerged as a generic and versatile method to control the activation of proteins quantitatively, quickly, and locally in a live organism. We present that approach and its uses in a variety of physiological contexts.

Original language	English
Pages (from-to)	1232-1238
Number of pages	7
Journal	ChemBioChem
Volume	19
Issue number	12
DOIs	https://doi.org/10.1002/cbic.201700630
State	Published - 18 Jun 2018
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim

Funding

D.B. and S.W. acknowledge support by CNRS under a LIA between CNRS and UCLA and by the Partner University Fund a program of the French American Culture Exchange. D.B. and L.J. acknowledge the support of an ITMO Cancer grant “PhotoCancer” and the Structuration de la Recherche PSL (ANR-10-IDEX-0001–02 PSL), Microgut and SuperLine grants. D.B., S.V., and M.V. acknowledge support under the program “Investissements d’Avenir” launched by the French Government and implemented by the ANR with the references: ANR-10-LABX-54 MEMO LIFE and ANR-11-IDEX-0001–02 PSL* Research University. L.J. acknowledges ANR (France BioImaging-ANR-10-INBS-04, Morphoscope2-ANR-11-EQPX-0029). Z.F. was supported by Dean Willard Chair fund, CSC scholarship and UCLA Dissertation Year fellowship. S.Y. acknowledges the support of ANR-JCJC and the National Natural Science Foundation of China (31528007). D.B. and S.W. acknowledge support by CNRS under a LIA between CNRS and UCLA and by the Partner University Fund a program of the French American Culture Exchange. D.B. and L.J. acknowledge the support of an ITMO Cancer grant ?PhotoCancer? and the Structuration de la Recherche PSL (ANR-10-IDEX-0001?02 PSL), Microgut and SuperLine grants. D.B., S.V., and M.V. acknowledge support under the program ?Investissements d'Avenir? launched by the French Government and implemented by the ANR with the references: ANR-10-LABX-54 MEMO LIFE and ANR-11-IDEX-0001?02 PSL* Research University. L.J. acknowledges ANR (France BioImaging-ANR-10-INBS-04, Morphoscope2-ANR-11-EQPX-0029). Z.F. was supported by Dean Willard Chair fund, CSC scholarship and UCLA Dissertation Year fellowship. S.Y. acknowledges the support of ANR-JCJC and the National Natural Science Foundation of China (31528007).

Funders	Funder number
ANR-JCJC
French American Culture Exchange
ITMO
Structuration de la Recherche PSL	ANR-10-LABX-54 MEMO LIFE, ANR-11-IDEX-0001–02 PSL, ANR-10-IDEX-0001–02 PSL
University of California, Los Angeles
National Natural Science Foundation of China	31528007
China Scholarship Council
Centre National de la Recherche Scientifique
ITMO University

Keywords

caged compounds
cyclofen-OH
gene expression
optogenetics
photolysis

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10.1002/cbic.201700630

Cite this

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abstract = "The use of light to control the expression of genes and the activity of proteins is a rapidly expanding field. Whereas many of these approaches use fusion between a light-activable protein and the protein of interest to control the activity of the latter, it is also possible to control the activity of a protein by uncaging a specific ligand. In that context, controlling the activation of a protein fused to the modified estrogen receptor (ERT) by uncaging its ligand cyclofen-OH has emerged as a generic and versatile method to control the activation of proteins quantitatively, quickly, and locally in a live organism. We present that approach and its uses in a variety of physiological contexts.",

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AU - Ye, Shixin

AU - Weiss, Shimon

AU - Volovitch, Michel

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AU - Jullien, Ludovic

AU - Bensimon, David

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Control of Protein Activity and Gene Expression by Cyclofen-OH Uncaging

Abstract

Bibliographical note

Funding

Keywords

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