Abstract
The use of light to control the expression of genes and the activity of proteins is a rapidly expanding field. Whereas many of these approaches use fusion between a light-activable protein and the protein of interest to control the activity of the latter, it is also possible to control the activity of a protein by uncaging a specific ligand. In that context, controlling the activation of a protein fused to the modified estrogen receptor (ERT) by uncaging its ligand cyclofen-OH has emerged as a generic and versatile method to control the activation of proteins quantitatively, quickly, and locally in a live organism. We present that approach and its uses in a variety of physiological contexts.
| Original language | English |
|---|---|
| Pages (from-to) | 1232-1238 |
| Number of pages | 7 |
| Journal | ChemBioChem |
| Volume | 19 |
| Issue number | 12 |
| DOIs | |
| State | Published - 18 Jun 2018 |
| Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
Funding
D.B. and S.W. acknowledge support by CNRS under a LIA between CNRS and UCLA and by the Partner University Fund a program of the French American Culture Exchange. D.B. and L.J. acknowledge the support of an ITMO Cancer grant “PhotoCancer” and the Structuration de la Recherche PSL (ANR-10-IDEX-0001–02 PSL), Microgut and SuperLine grants. D.B., S.V., and M.V. acknowledge support under the program “Investissements d’Avenir” launched by the French Government and implemented by the ANR with the references: ANR-10-LABX-54 MEMO LIFE and ANR-11-IDEX-0001–02 PSL* Research University. L.J. acknowledges ANR (France BioImaging-ANR-10-INBS-04, Morphoscope2-ANR-11-EQPX-0029). Z.F. was supported by Dean Willard Chair fund, CSC scholarship and UCLA Dissertation Year fellowship. S.Y. acknowledges the support of ANR-JCJC and the National Natural Science Foundation of China (31528007). D.B. and S.W. acknowledge support by CNRS under a LIA between CNRS and UCLA and by the Partner University Fund a program of the French American Culture Exchange. D.B. and L.J. acknowledge the support of an ITMO Cancer grant ?PhotoCancer? and the Structuration de la Recherche PSL (ANR-10-IDEX-0001?02 PSL), Microgut and SuperLine grants. D.B., S.V., and M.V. acknowledge support under the program ?Investissements d'Avenir? launched by the French Government and implemented by the ANR with the references: ANR-10-LABX-54 MEMO LIFE and ANR-11-IDEX-0001?02 PSL* Research University. L.J. acknowledges ANR (France BioImaging-ANR-10-INBS-04, Morphoscope2-ANR-11-EQPX-0029). Z.F. was supported by Dean Willard Chair fund, CSC scholarship and UCLA Dissertation Year fellowship. S.Y. acknowledges the support of ANR-JCJC and the National Natural Science Foundation of China (31528007).
| Funders | Funder number |
|---|---|
| ANR-JCJC | |
| French American Culture Exchange | |
| ITMO | |
| Structuration de la Recherche PSL | ANR-10-LABX-54 MEMO LIFE, ANR-11-IDEX-0001–02 PSL, ANR-10-IDEX-0001–02 PSL |
| University of California, Los Angeles | |
| National Natural Science Foundation of China | 31528007 |
| China Scholarship Council | |
| Centre National de la Recherche Scientifique | |
| ITMO University |
Keywords
- caged compounds
- cyclofen-OH
- gene expression
- optogenetics
- photolysis