Contact Order Is a Determinant for the Dependence of GFP Folding on the Chaperonin GroEL

Boudhayan Bandyopadhyay, Tridib Mondal, Ron Unger, Amnon Horovitz

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The GroE chaperonin system facilitates protein folding in an ATP-dependent manner. It has remained unclear why some proteins are obligate clients of the GroE system, whereas other closely related proteins are able to fold efficiently in its absence. Factors that cause folding to be slower affect kinetic partitioning between spontaneous folding and chaperone binding in favor of the latter. One such potential factor is contact order (CO), which is the average separation in sequence between residues that are in contact in the native structure. Here, we generated variants of enhanced green fluorescent protein with different COs using circular permutations. We found that GroE dependence in vitro and in vivo increases with increasing CO. Thus, our results show that CO is relevant not only for folding in vitro of relatively simple model systems but also for chaperonin dependence and folding in vivo.

Original languageEnglish
Pages (from-to)42-48
Number of pages7
JournalBiophysical Journal
Volume116
Issue number1
DOIs
StatePublished - 8 Jan 2019

Bibliographical note

Publisher Copyright:
© 2018 Biophysical Society

Funding

This work was supported by the Minerva Foundation with funding from the Federal German Ministry for Education and Research (to A.H.), grant 2015170 of the US-Israel Binational Science Foundation (to A.H.), and grant 772/13 of the Israel Science Foundation (to R.U.). This work was supported by the Minerva Foundation with funding from the Federal German Ministry for Education and Research (to A.H.), grant 2015170 of the US-Israel Binational Science Foundation (to A.H.), and grant 772/13 of the Israel Science Foundation (to R.U.).

FundersFunder number
Federal German Ministry for Education and Research2015170
US-Israel Binational Science Foundation
Minerva Foundation
United States-Israel Binational Science Foundation772/13
Israel Science Foundation

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