Constructing protein polyhedra via orthogonal chemical interactions

Eyal Golub, Rohit H. Subramanian, Julian Esselborn, Robert G. Alberstein, Jake B. Bailey, Jerika A. Chiong, Xiaodong Yan, Timothy Booth, Timothy S. Baker, F. Akif Tezcan

Research output: Contribution to journalArticlepeer-review

86 Scopus citations


Many proteins exist naturally as symmetrical homooligomers or homopolymers1. The emergent structural and functional properties of such protein assemblies have inspired extensive efforts in biomolecular design2–5. As synthesized by ribosomes, proteins are inherently asymmetric. Thus, they must acquire multiple surface patches that selectively associate to generate the different symmetry elements needed to form higher-order architectures1,6—a daunting task for protein design. Here we address this problem using an inorganic chemical approach, whereby multiple modes of protein–protein interactions and symmetry are simultaneously achieved by selective, ‘one-pot’ coordination of soft and hard metal ions. We show that a monomeric protein (protomer) appropriately modified with biologically inspired hydroxamate groups and zinc-binding motifs assembles through concurrent Fe3+ and Zn2+ coordination into discrete dodecameric and hexameric cages. Our cages closely resemble natural polyhedral protein architectures7,8 and are, to our knowledge, unique among designed systems9–13 in that they possess tightly packed shells devoid of large apertures. At the same time, they can assemble and disassemble in response to diverse stimuli, owing to their heterobimetallic construction on minimal interprotein-bonding footprints. With stoichiometries ranging from [2 Fe:9 Zn:6 protomers] to [8 Fe:21 Zn:12 protomers], these protein cages represent some of the compositionally most complex protein assemblies—or inorganic coordination complexes—obtained by design.

Original languageEnglish
Pages (from-to)172-176
Number of pages5
Issue number7793
StatePublished - 6 Feb 2020
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2020, The Author(s), under exclusive licence to Springer Nature Limited.


Acknowledgements This work was supported by the US Department of Energy (Division of Materials Sciences, Office of Basic Energy Sciences; DE-SC0003844; for the design strategy, EM imaging and analysis, and biochemical analysis) and by the National Science Foundation (Division of Materials Research; DMR-1602537; for crystallographic analysis). E.G. acknowledges support by an EMBO Long-Term Postdoctoral Fellowship (ALTF 1336-2015). J.E. acknowledges support by a DFG Research Fellowship (DFG 393131496). R.H.S. was supported by the National Institute of Health Chemical Biology Interfaces Training Grant UC San Diego (T32GM112584). We acknowledge the use of the UCSD Cryo-EM Facility, which is supported by NIH grants to T.S.B. and a gift from the Agouron Institute to UCSD. Crystallographic data were collected either at Stanford Synchrotron Radiation Lightsource (SSRL) or at the Lawrence Berkeley Natural Laboratory on behalf of the Department of Energy.

FundersFunder number
National Institute of Health Chemical Biology Interfaces Training Grant UC San DiegoT32GM112584
National Science Foundation
National Institutes of Health
U.S. Department of Energy
National Institute of General Medical SciencesR37GM033050
Division of Materials ResearchDMR-1602537
European Molecular Biology OrganizationALTF 1336-2015, DFG 393131496
Basic Energy SciencesDE-SC0003844
Agouron Institute


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