Condensin I recruitment and uneven chromatin condensation precede mitotic cell death in response to DNA damage

Michael Blank, Yaniv Lerenthal, Leonid Mittelman, Yosef Shiloh

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Mitotic cell death (MCD) is a prominent but poorly defined form of death that stems from aberrant mitosis. One of the early steps in MCD is premature mitosis and uneven chromatin condensation (UCC). The mechanism underlying this phenomenon is currently unknown. In this study, we show that DNA damage in cells with a compromised p53-mediated G2/M checkpoint triggers the unscheduled activation of cyclin-dependent kinase 1 (Cdk1), activation and chromatin loading of the condensin I complex, and UCC followed by the appearance of multimicronucleated cells, which is evidence of MCD. We demonstrate that these processes engage some of the players of normal mitotic chromatin packaging but not those that drive the apoptotic chromatin condensation. Our findings establish a link between the induction of DNA damage and mitotic abnormalities (UCC) through the unscheduled activation of Cdk1 and recruitment of condensin I. These results demonstrate a clear distinction between the mechanisms that drive MCD-associated and apoptosis-related chromatin condensation and provide mechanistic insights and new readouts for a major cell death process in treated tumors.

Original languageEnglish
Pages (from-to)195-206
Number of pages12
JournalJournal of Cell Biology
Volume174
Issue number2
DOIs
StatePublished - 17 Jul 2006
Externally publishedYes

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