Computed tomography for in vivo deep over-1000 nm near-infrared fluorescence imaging

Masakazu Umezawa; Toshihiro Sera; Hideo Yokota; Maho Takematsu; Masahiko Morita; Gil Yeroslavsky; Masao Kamimura; Kohei Soga

doi:10.1002/jbio.202000071

Computed tomography for in vivo deep over-1000 nm near-infrared fluorescence imaging

Masakazu Umezawa, Toshihiro Sera, Hideo Yokota, Maho Takematsu, Masahiko Morita, Gil Yeroslavsky, Masao Kamimura, Kohei Soga

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

This study aims to develop a novel cross-sectional imaging of fluorescence in over-1000 nm near-infrared (OTN-NIR), which allows in vivo deep imaging, using computed tomography (CT) system. Cylindrical specimens of composite of OTN-NIR fluorophore, NaGdF₄ co-doped with Yb³⁺ and Ho³⁺ (ex: 980 nm, em: 1150 nm), were embedded in cubic agar (10.5–12 mm) or in the peritoneal cavity of mice and placed on a rotatable stage. When the fluorescence from inside of the samples was serially captured from multiple angles, the images were disrupted by the reflection and refraction of emitted light on the sample-air interface. Immersing the sample into water filled in a rectangular bath suppressed the disruption at the interface and successfully reconstructed the position and concentration of OTN-NIR fluorophores on the cross-sectional images using a CT technique. This is promising as a novel three-dimensional imaging technique for OTN-NIR fluorescent image projections of small animals captured from multiple angles.

Original language	English
Article number	e202000071
Journal	Journal of Biophotonics
Volume	13
Issue number	8
DOIs	https://doi.org/10.1002/jbio.202000071
State	Published - 1 Aug 2020
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Funding

We thank the graduate and undergraduate students for their technical assistance in the Soga Laboratory in Tokyo University of Science, especially Mr. Soichiro Fujii and Mr. Takeaki Harada for optical system setup, Mr. Ryuta Takeda and Mr. Takaya Yamada for preparation of NaGdF co‐doped with Yb and Ho, and Ms. Yuki Uenomachi for optimizing agarose gel‐based biological phantom. This work was supported in part by the MEXT Grant‐in‐Aid for Scientific Research on Innovative Areas (Resonance Bio), no. 15H05950, the MEXT Grant‐in‐Aid for Scientific Research (A), no. 19H01179, the MEXT‐Supported Program for the Strategic Research Foundation at Private Universities, no. S1511012, and the Center of Innovation Program “COINS” from Japan Science and Technology Agency, JST. 4 3+ 3+ Ministry of Education, Culture, Sports, Science and Technology, Grant/Award Numbers: S1511012, 19H01179, 15H05950; Japan Science and Technology Agency, Grant/Award Number: Center of Innovation Program “COINS” Funding information We thank the graduate and undergraduate students for their technical assistance in the Soga Laboratory in Tokyo University of Science, especially Mr. Soichiro Fujii and Mr. Takeaki Harada for optical system setup, Mr. Ryuta Takeda and Mr. Takaya Yamada for preparation of NaGdF4 co-doped with Yb3+ and Ho3+, and Ms. Yuki Uenomachi for optimizing agarose gel-based biological phantom. This work was supported in part by the MEXT Grant-in-Aid for Scientific Research on Innovative Areas (Resonance Bio), no. 15H05950, the MEXT Grant-in-Aid for Scientific Research (A), no. 19H01179, the MEXT-Supported Program for the Strategic Research Foundation at Private Universities, no. S1511012, and the Center of Innovation Program ?COINS? from Japan Science and Technology Agency, JST.

Funders	Funder number
MEXT-Supported
Ministry of Education, Culture, Sports, Science and Technology	15H05950, 19H01179, S1511012
Japan Science and Technology Agency
University of Tokyo

Keywords

computed tomography
fluorescence
near infrared
three-dimensional imaging

Access to Document

10.1002/jbio.202000071

Cite this

@article{e8d00286ca654138a382de47e215cffd,

title = "Computed tomography for in vivo deep over-1000 nm near-infrared fluorescence imaging",

abstract = "This study aims to develop a novel cross-sectional imaging of fluorescence in over-1000 nm near-infrared (OTN-NIR), which allows in vivo deep imaging, using computed tomography (CT) system. Cylindrical specimens of composite of OTN-NIR fluorophore, NaGdF4 co-doped with Yb3+ and Ho3+ (ex: 980 nm, em: 1150 nm), were embedded in cubic agar (10.5–12 mm) or in the peritoneal cavity of mice and placed on a rotatable stage. When the fluorescence from inside of the samples was serially captured from multiple angles, the images were disrupted by the reflection and refraction of emitted light on the sample-air interface. Immersing the sample into water filled in a rectangular bath suppressed the disruption at the interface and successfully reconstructed the position and concentration of OTN-NIR fluorophores on the cross-sectional images using a CT technique. This is promising as a novel three-dimensional imaging technique for OTN-NIR fluorescent image projections of small animals captured from multiple angles.",

keywords = "computed tomography, fluorescence, near infrared, three-dimensional imaging",

author = "Masakazu Umezawa and Toshihiro Sera and Hideo Yokota and Maho Takematsu and Masahiko Morita and Gil Yeroslavsky and Masao Kamimura and Kohei Soga",

note = "Publisher Copyright: {\textcopyright} 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim",

year = "2020",

month = aug,

day = "1",

doi = "10.1002/jbio.202000071",

language = "אנגלית",

volume = "13",

journal = "Journal of Biophotonics",

issn = "1864-063X",

publisher = "Wiley-VCH Verlag",

number = "8",

}

TY - JOUR

T1 - Computed tomography for in vivo deep over-1000 nm near-infrared fluorescence imaging

AU - Umezawa, Masakazu

AU - Sera, Toshihiro

AU - Yokota, Hideo

AU - Takematsu, Maho

AU - Morita, Masahiko

AU - Yeroslavsky, Gil

AU - Kamimura, Masao

AU - Soga, Kohei

PY - 2020/8/1

Y1 - 2020/8/1

N2 - This study aims to develop a novel cross-sectional imaging of fluorescence in over-1000 nm near-infrared (OTN-NIR), which allows in vivo deep imaging, using computed tomography (CT) system. Cylindrical specimens of composite of OTN-NIR fluorophore, NaGdF4 co-doped with Yb3+ and Ho3+ (ex: 980 nm, em: 1150 nm), were embedded in cubic agar (10.5–12 mm) or in the peritoneal cavity of mice and placed on a rotatable stage. When the fluorescence from inside of the samples was serially captured from multiple angles, the images were disrupted by the reflection and refraction of emitted light on the sample-air interface. Immersing the sample into water filled in a rectangular bath suppressed the disruption at the interface and successfully reconstructed the position and concentration of OTN-NIR fluorophores on the cross-sectional images using a CT technique. This is promising as a novel three-dimensional imaging technique for OTN-NIR fluorescent image projections of small animals captured from multiple angles.

AB - This study aims to develop a novel cross-sectional imaging of fluorescence in over-1000 nm near-infrared (OTN-NIR), which allows in vivo deep imaging, using computed tomography (CT) system. Cylindrical specimens of composite of OTN-NIR fluorophore, NaGdF4 co-doped with Yb3+ and Ho3+ (ex: 980 nm, em: 1150 nm), were embedded in cubic agar (10.5–12 mm) or in the peritoneal cavity of mice and placed on a rotatable stage. When the fluorescence from inside of the samples was serially captured from multiple angles, the images were disrupted by the reflection and refraction of emitted light on the sample-air interface. Immersing the sample into water filled in a rectangular bath suppressed the disruption at the interface and successfully reconstructed the position and concentration of OTN-NIR fluorophores on the cross-sectional images using a CT technique. This is promising as a novel three-dimensional imaging technique for OTN-NIR fluorescent image projections of small animals captured from multiple angles.

KW - computed tomography

KW - fluorescence

KW - near infrared

KW - three-dimensional imaging

UR - http://www.scopus.com/inward/record.url?scp=85086036497&partnerID=8YFLogxK

U2 - 10.1002/jbio.202000071

DO - 10.1002/jbio.202000071

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 32388908

AN - SCOPUS:85086036497

SN - 1864-063X

VL - 13

JO - Journal of Biophotonics

JF - Journal of Biophotonics

IS - 8

M1 - e202000071

ER -

Computed tomography for in vivo deep over-1000 nm near-infrared fluorescence imaging

Abstract

Bibliographical note

Funding

Keywords

Access to Document

Other files and links

Fingerprint

Cite this