Abstract
Acanthamoeba keratitis (AK), a potentially blinding infection of the cornea, is caused by a free-living protozoan. Culture and microscopic examination of corneal scraping tissue material is the conventional method for identifying Acanthamoeba. In this article, we compared several methods for AK diagnosis of 32 patients: microscopic examination using fluorescent dye, specific culture on growth media-non-nutrient agar (NNA), culture on liquid growth media-peptone yeast glucose (PYG), and TYI-S-33. AK was found in 14 patients. Thirteen of the specimens were found AK positive by fluorescence microscopic examination, 11 specimens were found AK positive on PYG growth media, and 9 specimens were found AK positive on TYI-S-33 growth media. Only five specimens were found AK positive on NNA growth media. Therefore, we recommend using fluorescence microscopy technique and culture method, especially PYG liquid media.
| Original language | English |
|---|---|
| Pages (from-to) | 316-318 |
| Number of pages | 3 |
| Journal | American Journal of Tropical Medicine and Hygiene |
| Volume | 93 |
| Issue number | 2 |
| DOIs | |
| State | Published - 1 Aug 2015 |
| Externally published | Yes |
Bibliographical note
Publisher Copyright:© 2015 by The American Society of Tropical Medicine and Hygiene.
UN SDGs
This output contributes to the following UN Sustainable Development Goals (SDGs)
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SDG 3 Good Health and Well-being
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