TY - JOUR
T1 - Comparative phytochemical and antioxidant analysis in leaf and bark of Symplocos cochinchinensis (Lour.) Moore ssp. laurina (Retz.) Nooteb
AU - Ayyathan, Dhanoop Manikoth
AU - Krishna, Archana
AU - Mathews, Shama Elza
AU - Chandrasekaran, Rajaseakran
AU - Thiagarajan, Kalaivani
PY - 2015
Y1 - 2015
N2 - Medicinal plants are widely used for therapeutic purposes as their side effects are comparatively less. The genus Symplocos belonging to the family Symplocaceae comprises of 300-500 species. The present study was to evaluate phytochemical constitution and antioxidant activity of ethanolic leaf and bark extracts of S. laurina (L.) for understanding, therapeutic effects of the plant for the first time. Phytochemical constituents like total phenols, flavonoids and alkaloids were also determined in this study. Our results demonstrated that the ethanolic extracts of S. laurina (L.) had more flavonoid content in leaf (360 mg) than bark (70 mg) and also the total phenolics content in the ethanolic leaf extract of S. laurina (L.) showed more than bark (720 mg and 180 mg respectively). Also the free radical scavenging properties of these extracts were compared by DPPH assay and found significant inhibition were exhibited depending on the concentration, IC50 value in both the leaf and bark were observed 500 μg/mL. With increasing concentrations ethanolic leaf extracts of S. laurina (L.) showed more reducing activity compared to bark at 10 μg/mL. The HPLC analysis showed the presence of ß-sitosterol in ethanolic leaf and bark extracts of S. laurina (L.).
AB - Medicinal plants are widely used for therapeutic purposes as their side effects are comparatively less. The genus Symplocos belonging to the family Symplocaceae comprises of 300-500 species. The present study was to evaluate phytochemical constitution and antioxidant activity of ethanolic leaf and bark extracts of S. laurina (L.) for understanding, therapeutic effects of the plant for the first time. Phytochemical constituents like total phenols, flavonoids and alkaloids were also determined in this study. Our results demonstrated that the ethanolic extracts of S. laurina (L.) had more flavonoid content in leaf (360 mg) than bark (70 mg) and also the total phenolics content in the ethanolic leaf extract of S. laurina (L.) showed more than bark (720 mg and 180 mg respectively). Also the free radical scavenging properties of these extracts were compared by DPPH assay and found significant inhibition were exhibited depending on the concentration, IC50 value in both the leaf and bark were observed 500 μg/mL. With increasing concentrations ethanolic leaf extracts of S. laurina (L.) showed more reducing activity compared to bark at 10 μg/mL. The HPLC analysis showed the presence of ß-sitosterol in ethanolic leaf and bark extracts of S. laurina (L.).
KW - Alkaloids
KW - Antioxidants
KW - Flavonoids
KW - Phenols
KW - S. laurina (L.)
UR - http://www.scopus.com/inward/record.url?scp=84921447136&partnerID=8YFLogxK
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AN - SCOPUS:84921447136
SN - 0975-8585
VL - 6
SP - 292
EP - 299
JO - Research Journal of Pharmaceutical, Biological and Chemical Sciences
JF - Research Journal of Pharmaceutical, Biological and Chemical Sciences
IS - 1
ER -