Comparative application of microwave, ultrasonication, ultracentrifugation and conventional heating for preparation of sample as dinitrophenyl derivative for direct enantioseparation of certain amino alcohols and 1-amino-2-propanol from vitamin B12 hydrolysate on α1-acid glycoprotein and β-cyclodextrin columns

Ravi Bhushan; Rajender Kumar

doi:10.1016/j.chroma.2009.09.049

Comparative application of microwave, ultrasonication, ultracentrifugation and conventional heating for preparation of sample as dinitrophenyl derivative for direct enantioseparation of certain amino alcohols and 1-amino-2-propanol from vitamin B₁₂ hydrolysate on α₁-acid glycoprotein and β-cyclodextrin columns

Ravi Bhushan, Rajender Kumar

Indian Institute of Technology Roorkee

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Taking into account the structural similarities of amino alcohols with amino acids and in order to reduce time for derivatization unconventional approaches viz. microwave irradiation, ultrasonication and ultra centrifugation were applied for synthesis of dinitrophenyl derivatives of nine amino alcohols and to work out a method of choice for sample preparation for direct enantioseparation. The enantiomeric dinitrophenyl derivatives so synthesized were separated on α₁-acid glycoprotein and β-cyclodextrin columns with detection at 230 nm using photodiode array detection system. Derivatization methods and chromatographic parameters were optimized. β-Cyclodextrin column was found better compared to AGP column for enantioseparation. Limit of detection, quantification, accuracy and precision were also determined. The developed method was successfully applied to determine enantiomeric purity of 1-amino-2-propanol obtained from vitamin B₁₂ hydrolysate.

Original language	English
Pages (from-to)	7941-7945
Number of pages	5
Journal	Journal of Chromatography A
Volume	1216
Issue number	45
DOIs	https://doi.org/10.1016/j.chroma.2009.09.049
State	Published - 6 Nov 2009
Externally published	Yes

Bibliographical note

Funding Information:
The authors are thankful to Council of Scientific and Industrial Research (CSIR), New Delhi, India, for award of a senior research fellowship to (R.K.). Thanks are due to Alexander von Humboldt Foundation, Bonn, Germany, for donating Knauer HPLC equipment (to RB in 2006) and to Professor Hans Brückner (IFZ, University of Giessen, Germany) for donating homophenyl alaninol used in the present studies.

Funding

The authors are thankful to Council of Scientific and Industrial Research (CSIR), New Delhi, India, for award of a senior research fellowship to (R.K.). Thanks are due to Alexander von Humboldt Foundation, Bonn, Germany, for donating Knauer HPLC equipment (to RB in 2006) and to Professor Hans Brückner (IFZ, University of Giessen, Germany) for donating homophenyl alaninol used in the present studies.

Funders	Funder number
Council of Scientific and Industrial Research, India

Keywords

AGP
Direct chiral separation
Fluorodinitrobenzene
High performance liquid chromatography
β-Amino alcohols
β-Cyclodextrin

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10.1016/j.chroma.2009.09.049

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Bhushan, R., & Kumar, R. (2009). Comparative application of microwave, ultrasonication, ultracentrifugation and conventional heating for preparation of sample as dinitrophenyl derivative for direct enantioseparation of certain amino alcohols and 1-amino-2-propanol from vitamin B₁₂ hydrolysate on α₁-acid glycoprotein and β-cyclodextrin columns. Journal of Chromatography A, 1216(45), 7941-7945. https://doi.org/10.1016/j.chroma.2009.09.049

Bhushan, Ravi ; Kumar, Rajender. / Comparative application of microwave, ultrasonication, ultracentrifugation and conventional heating for preparation of sample as dinitrophenyl derivative for direct enantioseparation of certain amino alcohols and 1-amino-2-propanol from vitamin B₁₂ hydrolysate on α₁-acid glycoprotein and β-cyclodextrin columns. In: Journal of Chromatography A. 2009 ; Vol. 1216, No. 45. pp. 7941-7945.

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abstract = "Taking into account the structural similarities of amino alcohols with amino acids and in order to reduce time for derivatization unconventional approaches viz. microwave irradiation, ultrasonication and ultra centrifugation were applied for synthesis of dinitrophenyl derivatives of nine amino alcohols and to work out a method of choice for sample preparation for direct enantioseparation. The enantiomeric dinitrophenyl derivatives so synthesized were separated on α1-acid glycoprotein and β-cyclodextrin columns with detection at 230 nm using photodiode array detection system. Derivatization methods and chromatographic parameters were optimized. β-Cyclodextrin column was found better compared to AGP column for enantioseparation. Limit of detection, quantification, accuracy and precision were also determined. The developed method was successfully applied to determine enantiomeric purity of 1-amino-2-propanol obtained from vitamin B12 hydrolysate.",

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Bhushan, R & Kumar, R 2009, 'Comparative application of microwave, ultrasonication, ultracentrifugation and conventional heating for preparation of sample as dinitrophenyl derivative for direct enantioseparation of certain amino alcohols and 1-amino-2-propanol from vitamin B₁₂ hydrolysate on α₁-acid glycoprotein and β-cyclodextrin columns', Journal of Chromatography A, vol. 1216, no. 45, pp. 7941-7945. https://doi.org/10.1016/j.chroma.2009.09.049

Comparative application of microwave, ultrasonication, ultracentrifugation and conventional heating for preparation of sample as dinitrophenyl derivative for direct enantioseparation of certain amino alcohols and 1-amino-2-propanol from vitamin B₁₂ hydrolysate on α₁-acid glycoprotein and β-cyclodextrin columns. / Bhushan, Ravi; Kumar, Rajender.
In: Journal of Chromatography A, Vol. 1216, No. 45, 06.11.2009, p. 7941-7945.

Research output: Contribution to journal › Article › peer-review

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AB - Taking into account the structural similarities of amino alcohols with amino acids and in order to reduce time for derivatization unconventional approaches viz. microwave irradiation, ultrasonication and ultra centrifugation were applied for synthesis of dinitrophenyl derivatives of nine amino alcohols and to work out a method of choice for sample preparation for direct enantioseparation. The enantiomeric dinitrophenyl derivatives so synthesized were separated on α1-acid glycoprotein and β-cyclodextrin columns with detection at 230 nm using photodiode array detection system. Derivatization methods and chromatographic parameters were optimized. β-Cyclodextrin column was found better compared to AGP column for enantioseparation. Limit of detection, quantification, accuracy and precision were also determined. The developed method was successfully applied to determine enantiomeric purity of 1-amino-2-propanol obtained from vitamin B12 hydrolysate.

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Bhushan R, Kumar R. Comparative application of microwave, ultrasonication, ultracentrifugation and conventional heating for preparation of sample as dinitrophenyl derivative for direct enantioseparation of certain amino alcohols and 1-amino-2-propanol from vitamin B₁₂ hydrolysate on α₁-acid glycoprotein and β-cyclodextrin columns. Journal of Chromatography A. 2009 Nov 6;1216(45):7941-7945. doi: 10.1016/j.chroma.2009.09.049