Common-path phase-shifting digital holographic microscopy: A way to quantitative phase imaging and superresolution

Vicente Mico, Zeev Zalevsky, Javier García

Research output: Contribution to journalArticlepeer-review

88 Scopus citations

Abstract

We present an experimental setup useful for complex amplitude evaluation and phase image quantification of three-dimensional (3-D) samples in digital holographic microscopy (DHM). It is based on a common-path interferometric configuration performed by dividing the input plane in two contiguous regions and by placing a translation grating near to the Fourier plane. Then, complex amplitude distribution of the sample under test is recovered with phase-shifting standard method obtained by moving the grating using a linear motion stage. Some experimental results of an USAF resolution test are presented for different numerical aperture (NA) microscope lenses. In a second part, the proposed setup is tested under superresolution purposes. Based on the object's spectrum shift produced by off-axis illumination, we use time multiplexing to generate a synthetic aperture enlargement that improves the final image resolution. Experimental results for the case of a biosample (human red blood cells) and a commercial low NA microscope lens validates the suggested superresolution approach.

Original languageEnglish
Pages (from-to)4273-4281
Number of pages9
JournalOptics Communications
Volume281
Issue number17
DOIs
StatePublished - 1 Sep 2008

Bibliographical note

Funding Information:
The authors want to thank Professor Francisco Javier Perucho Lizcano for the human red blood cells biosample preparation. Also, part of this work was supported by the Spanish Ministerio de Educación y Ciencia and FEDER funds under the Project FIS2007-60626.

Keywords

  • Digital holographic microscopy
  • Fourier image formation
  • Phase-shifting techniques
  • Quantitative phase imaging
  • Superresolution
  • Synthetic aperture microscopy

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