TY - JOUR
T1 - Commercial human 3D corneal epithelial equivalents for modeling epithelial infection in herpes keratitis
AU - Borodianskiy-Shteinberg, Tatiana
AU - Bisht, Punam
AU - Das, Biswajit
AU - Kinchington, Paul R.
AU - Goldstein, Ronald S.
N1 - Publisher Copyright:
© 2024 Elsevier Inc.
PY - 2024/7
Y1 - 2024/7
N2 - Herpes stromal keratitis is the leading cause of infectious blindness in the western world. Infection by HSV1 is most common, but VZV and hCMV also infect the cornea. Multiple models of HSV1 corneal infection exist, but none for VZV and hCMV because of their host specificity. Here, we used commercially available 3D human corneal epithelial equivalents (HCEE) to study infection by these herpesviruses. HCEE was infected by HSV-1 and hCMV without requiring scarification and resulted in spreading infections. Spread of HSV-1 infection was rapid, while that of hCMV was slow. In contrast, infections with VZV required damage to the HCEE and did not spread. Acyclovir dramatically reduced replication of HSV-1 in this model. We conclude that highly quality-controlled, readily available HCEE is a useful model to study human-restricted herpesvirus infection of the human corneal epithelium and for screening of antiviral drugs for treating HSK in an 3D model system.
AB - Herpes stromal keratitis is the leading cause of infectious blindness in the western world. Infection by HSV1 is most common, but VZV and hCMV also infect the cornea. Multiple models of HSV1 corneal infection exist, but none for VZV and hCMV because of their host specificity. Here, we used commercially available 3D human corneal epithelial equivalents (HCEE) to study infection by these herpesviruses. HCEE was infected by HSV-1 and hCMV without requiring scarification and resulted in spreading infections. Spread of HSV-1 infection was rapid, while that of hCMV was slow. In contrast, infections with VZV required damage to the HCEE and did not spread. Acyclovir dramatically reduced replication of HSV-1 in this model. We conclude that highly quality-controlled, readily available HCEE is a useful model to study human-restricted herpesvirus infection of the human corneal epithelium and for screening of antiviral drugs for treating HSK in an 3D model system.
KW - 3D culture
KW - Drug testing
KW - Herpes keratitis
KW - Herpes zoster ophthalmicus
KW - Herpesvirus
UR - http://www.scopus.com/inward/record.url?scp=85191987595&partnerID=8YFLogxK
U2 - 10.1016/j.virol.2024.110096
DO - 10.1016/j.virol.2024.110096
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C2 - 38710129
AN - SCOPUS:85191987595
SN - 0042-6822
VL - 595
JO - Virology
JF - Virology
M1 - 110096
ER -