Cloning, expression and purification of the outer membrane protein28 of salmonella enterica serovar typhimurium for subunit vaccine development-a short communication

Translated title of the contribution: Cloning, expression and purification of the outer membrane protein28 of salmonella enterica serovar typhimurium for subunit vaccine development-a short communication

Mamta Pandey, Mumtesh K. Saxena, Anjani Saxena, Richa Jha, Sunil K. Rastogi, Rajesh Kumar

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Salmonella Typhimurium, a major gastrointestinal pathogen, poses a global threat to human health. Public health problems associated with this organism have increased to the extent that it has become a major issue. The bacterium is becoming resistant to the commonly available antibiotics, and vaccines also suffer from limitations such as short lived immunity. Therefore, there is an urgent need for the development of an effective vaccine. The outer membrane proteins (Omps) of Salmonella have proven their capability to be developed as a vaccine candidate for prevention of salmonellosis. With this aim, in the present study the Omp28 gene of Salmonella Typhimurium was amplified, cloned and expressed under an IPTG induction system. The recombinant protein thus produced was purified and tested for its antigenicity. The antigenicity of the purified protein was confirmed by western blotting with antiserum raised in rabbit against Omps of S. Typhimurium. The Omp28 gene was amplified as a 330bp product. The expressed protein was found to be of approximately 28kDa and it produced a strong signal in western blot analysis. This study concluded that Omp28 may be proven to be an effective candidate for the development of r-DNA vaccine against salmonellosis.

Translated title of the contributionCloning, expression and purification of the outer membrane protein28 of salmonella enterica serovar typhimurium for subunit vaccine development-a short communication
Original languageEnglish
Pages (from-to)559-568
Number of pages10
JournalVeterinarski Arhiv
Volume88
Issue number4
DOIs
StatePublished - 2018
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2018, University of Zagreb, Facultty of Veterinary Medicine. All rights reserved.

Keywords

  • Cloning
  • Expression
  • Omp28
  • Salmonella typhimurium
  • Vaccine
  • Western blot

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