Cloning and characterization of the tomato karyopherin α1 gene promoter

Liat Mizrachy, David Dabush, Yael Levy, Roni Aloni, Arie Altman, Yedidya Gafni

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


The karyopherin α1 (LeKAPα 1) gene of tomato (Lycopersicon esculentum) encodes a receptor involved in nuclear import. To analyze the expression pattern of this gene, a genomic clone containing its upstream region was isolated and sequenced. To study the promoter functionality, a 2170 bp fragment (LM1), was fused to glucuronidase (GUS) and introduced into petunia cells by particle bombardment. For further characterization of the promoter, one inverse and three deletion constructs were studied in cell suspension. To follow its expression in tobacco leaves, transgenic plants expressing GUS under the control of the LM1 promoter were made. Expression of LM1-GUS was largely restricted to actively growing leaf regions, suggesting possible involvement of active cell division and plant growth regulators in LeKAPα 1 expression.

Original languageEnglish
Pages (from-to)515-522
Number of pages8
JournalDevelopment Growth and Differentiation
Issue number6
StatePublished - Dec 2004
Externally publishedYes


  • Expression pattern
  • Kryopherin a
  • Plant growth regulators
  • Promoter analysis
  • Tobacco


Dive into the research topics of 'Cloning and characterization of the tomato karyopherin α1 gene promoter'. Together they form a unique fingerprint.

Cite this