Cloning and characterization of human RTVP-1b, a novel splice variant of RTVP-1 in glioma cells

Cunli Xiang, Ronit Sarid, Simona Cazacu, Susan Finniss, Hae Kyung Lee, Amotz Ziv-Av, Tom Mikkelsen, Chaya Brodie

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Here, we report the cloning and characterization of RTVP-1b, a novel splice variant of human RTVP-1, which was isolated from the U87 glioma cell line. Sequence analysis revealed that RTVP-1b contains an additional 71 base exon between exons 2 and 3 that is missing in RTVP-1, leading to a frame-shift and a different putative protein. The deduced protein was 237 amino acids in length, sharing the N-terminal 141 amino acids with RTVP-1. RT-PCR analysis demonstrated that RTVP-1b was expressed in a wide range of tissues and that its expression was different from that of RTVP-1. In contrast, RTVP-1 and RTVP-1b showed similar patterns of expression in astrocytic tumors; highly expressed in glioblastomas as compared to normal brains, low-grade astrocytomas and anaplastic oligodendrogliomas. Overexpression of RTVP-1b increased glioma cell proliferation but did not affect cell migration. Our results suggest that RTVP-1b represents a potential prognostic marker and therapeutic target in gliomas.

Original languageEnglish
Pages (from-to)612-618
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume362
Issue number3
DOIs
StatePublished - 26 Oct 2007

Bibliographical note

Funding Information:
This work was supported by CA-R21-96965 and R24 CA095809 from the National Cancer Institute and by the William and Karen Davidson Fund, Hermelin Brain Tumor Center.

Keywords

  • Glioma
  • Oligodendroglioma
  • Proliferation
  • RTVP-1
  • RTVP-1b
  • Splice variant

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