Abstract
Psendomonas aentginosa is an opportunistic human pathogen which infects and endangers the life of cystic fibrosis and other chronic patienis Avrumonas hydrophila is in genera! a cold-blood animal pathogen but is involved also in human infections The microenvironment in tissues of such patients may contain high salt concentrations which can inhibit (he giowth of bacteria injecting them P. aerugwosa and A. hytirophiki have osmoprotective mechanisms. In the present study, the growth and virulence factoi production of these bacteria was examined under hyperosmotic conditions in the presence of the osmoprotectants choline and betaine (1 or 5 mM). It was found that P. twrugmosct PAO1 ATCC 15692 could use both compounds while A. hydrophila ATCC 7060 could only survive in the presence of betaine, probably due to the absence of the ability to convert choline into betaine /. ticnigiiiosa and A. hytii'ophilu were exposed to NaCl stress of 0 3 or 0 4 M and the effect of choline and betaine (both at 5 mM) on proiease, elastase and hcmolysin pioduction was studied In the case of P. acruginosa, choline and betaine were effective in restoiation of the above-mentioned virulence factors while hemolysin production by A. hydrophila was restored only by betaine.
| Original language | English |
|---|---|
| Pages (from-to) | A1272 |
| Journal | FASEB Journal |
| Volume | 11 |
| Issue number | 9 |
| State | Published - 1997 |