Choline acetyltransferase activity is increased in combined cultures of spinal cord and muscle cells from mice

Earl L. Giller, Bruce K. Schrier, Asher Shainberg, H. Ronald Fisk, Phillip G. Nelson

Research output: Contribution to journalArticlepeer-review

80 Scopus citations

Abstract

The activity of choline acetyltransferase was more than tenfold greater in combined cultures of spinal cord and muscle cells than in cultures of spinal cord cells alone. This increase was associated with the formation of functional neuromuscular junctions in culture. Counts of silver-stained cells and determinations of other enzyme activities indicated that the increased choline acetyltransferase activity was not due to nonspecific neuronal survival but reflected greater activity in the surviving neurons. Hence, muscle had a marked, highly specific trophic effect on the cholinergic neurons that innervated it.

Original languageEnglish
Pages (from-to)588-589
Number of pages2
JournalScience
Volume182
Issue number4112
DOIs
StatePublished - 1973
Externally publishedYes

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