TY - JOUR
T1 - Characterization of two insertion sequences, IS701 and IS702, from the cyanobacterium Calothrix species PCC 7601
AU - Mazel, D.
AU - Bernard, C.
AU - Schwarz, R.
AU - Castets, A. M.
AU - Houmard, J.
AU - de Marsac, N. Tandeau
PY - 1991/9
Y1 - 1991/9
N2 - We describe the characterization of two insertion elements, IS701 and IS702, isolated from Calothrix species PCC 7601. These insertion elements were cloned from spontaneous pigmentation mutants. Both show the characteristics of typical bacterial insertion sequences, i.e. they present long terminal inverted repeats and they duplicate target DNA upon insertion. These elements share no homology with the only other cyanobacterial insertion sequence described so far, IS891. At least 15 copies of IS701 and 9 copies of IS702 were detected by hybridization experiments in the Calothrix 7601 genome. Their occurrence in several cyanobacterial strains is also reported.
AB - We describe the characterization of two insertion elements, IS701 and IS702, isolated from Calothrix species PCC 7601. These insertion elements were cloned from spontaneous pigmentation mutants. Both show the characteristics of typical bacterial insertion sequences, i.e. they present long terminal inverted repeats and they duplicate target DNA upon insertion. These elements share no homology with the only other cyanobacterial insertion sequence described so far, IS891. At least 15 copies of IS701 and 9 copies of IS702 were detected by hybridization experiments in the Calothrix 7601 genome. Their occurrence in several cyanobacterial strains is also reported.
UR - http://www.scopus.com/inward/record.url?scp=0025878592&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2958.1991.tb02146.x
DO - 10.1111/j.1365-2958.1991.tb02146.x
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C2 - 1662761
AN - SCOPUS:0025878592
SN - 0950-382X
VL - 5
SP - 2165
EP - 2170
JO - Molecular Microbiology
JF - Molecular Microbiology
IS - 9
ER -