Abstract
Skin is one of the major tissues displaying chronic diabetic complications. We have studied glucose transport following stimulation with insulin and IGF-1 in cultured mouse keratinocytes. In proliferating cells, acute stimulation with insulin and IGF-1 increased glucose uptake. Insulin translocated glucose transporters 1 and 5, whereas IGF-1 translocated glucose transporters 2 and 3. With differentiation, glucose transporter 3 expression increased and the expression of glucose transporters 1, 2, and 5 decreased. No increase in glucose uptake was observed, however, following stimulation with either hormone. These results indicate that insulin and IGF-1 differentially regulate glucose uptake as well as expression and translocation of specific transporters in skin keratinocytes.
| Original language | English |
|---|---|
| Pages (from-to) | 949-954 |
| Number of pages | 6 |
| Journal | Journal of Investigative Dermatology |
| Volume | 115 |
| Issue number | 6 |
| DOIs | |
| State | Published - 2000 |
Bibliographical note
Funding Information:Supported in part by the Sorrell Foundation and a grant from the Israel Science Foundation funded by the Israel Academy of Sciences. This work was supported by a grant from the Smokeless Tobacco Research Council. EW is a recipient of a Career Development Award from the Juvenile Diabetes Foundation International. SRS is the incumbent of the Louis Fisher Chair in Cellular Pathology.
Keywords
- Glucose transport
- Glucose transporters
- Growth factors
- Insulin
- Keratinocytes
- Skin
