Characterization of glucose transport system in keratinocytes: Insulin and IGF-1 differentially affect specific transporters

Shlomzion Shen; Efrat Wertheimer; Sanford R. Sampson; Tamar Tennenbaum

doi:10.1046/j.1523-1747.2000.00161.x

Characterization of glucose transport system in keratinocytes: Insulin and IGF-1 differentially affect specific transporters

Shlomzion Shen, Efrat Wertheimer, Sanford R. Sampson, Tamar Tennenbaum

The Mina and Everard Goodman Faculty of Life Sciences

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Skin is one of the major tissues displaying chronic diabetic complications. We have studied glucose transport following stimulation with insulin and IGF-1 in cultured mouse keratinocytes. In proliferating cells, acute stimulation with insulin and IGF-1 increased glucose uptake. Insulin translocated glucose transporters 1 and 5, whereas IGF-1 translocated glucose transporters 2 and 3. With differentiation, glucose transporter 3 expression increased and the expression of glucose transporters 1, 2, and 5 decreased. No increase in glucose uptake was observed, however, following stimulation with either hormone. These results indicate that insulin and IGF-1 differentially regulate glucose uptake as well as expression and translocation of specific transporters in skin keratinocytes.

Original language	English
Pages (from-to)	949-954
Number of pages	6
Journal	Journal of Investigative Dermatology
Volume	115
Issue number	6
DOIs	https://doi.org/10.1046/j.1523-1747.2000.00161.x
State	Published - Dec 2000

Bibliographical note

Funding Information:
Supported in part by the Sorrell Foundation and a grant from the Israel Science Foundation funded by the Israel Academy of Sciences. This work was supported by a grant from the Smokeless Tobacco Research Council. EW is a recipient of a Career Development Award from the Juvenile Diabetes Foundation International. SRS is the incumbent of the Louis Fisher Chair in Cellular Pathology.

Funding

Supported in part by the Sorrell Foundation and a grant from the Israel Science Foundation funded by the Israel Academy of Sciences. This work was supported by a grant from the Smokeless Tobacco Research Council. EW is a recipient of a Career Development Award from the Juvenile Diabetes Foundation International. SRS is the incumbent of the Louis Fisher Chair in Cellular Pathology.

Funders	Funder number
Smokeless Tobacco Research Council
Sorrell Foundation
Juvenile Diabetes Research Foundation International
Israel Academy of Sciences and Humanities
Israel Science Foundation

Keywords

Glucose transport
Glucose transporters
Growth factors
Insulin
Keratinocytes
Skin

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1046/j.1523-1747.2000.00161.x

Cite this

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title = "Characterization of glucose transport system in keratinocytes: Insulin and IGF-1 differentially affect specific transporters",

abstract = "Skin is one of the major tissues displaying chronic diabetic complications. We have studied glucose transport following stimulation with insulin and IGF-1 in cultured mouse keratinocytes. In proliferating cells, acute stimulation with insulin and IGF-1 increased glucose uptake. Insulin translocated glucose transporters 1 and 5, whereas IGF-1 translocated glucose transporters 2 and 3. With differentiation, glucose transporter 3 expression increased and the expression of glucose transporters 1, 2, and 5 decreased. No increase in glucose uptake was observed, however, following stimulation with either hormone. These results indicate that insulin and IGF-1 differentially regulate glucose uptake as well as expression and translocation of specific transporters in skin keratinocytes.",

keywords = "Glucose transport, Glucose transporters, Growth factors, Insulin, Keratinocytes, Skin",

author = "Shlomzion Shen and Efrat Wertheimer and Sampson, {Sanford R.} and Tamar Tennenbaum",

note = "Funding Information: Supported in part by the Sorrell Foundation and a grant from the Israel Science Foundation funded by the Israel Academy of Sciences. This work was supported by a grant from the Smokeless Tobacco Research Council. EW is a recipient of a Career Development Award from the Juvenile Diabetes Foundation International. SRS is the incumbent of the Louis Fisher Chair in Cellular Pathology.",

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AU - Tennenbaum, Tamar

N1 - Funding Information: Supported in part by the Sorrell Foundation and a grant from the Israel Science Foundation funded by the Israel Academy of Sciences. This work was supported by a grant from the Smokeless Tobacco Research Council. EW is a recipient of a Career Development Award from the Juvenile Diabetes Foundation International. SRS is the incumbent of the Louis Fisher Chair in Cellular Pathology.

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N2 - Skin is one of the major tissues displaying chronic diabetic complications. We have studied glucose transport following stimulation with insulin and IGF-1 in cultured mouse keratinocytes. In proliferating cells, acute stimulation with insulin and IGF-1 increased glucose uptake. Insulin translocated glucose transporters 1 and 5, whereas IGF-1 translocated glucose transporters 2 and 3. With differentiation, glucose transporter 3 expression increased and the expression of glucose transporters 1, 2, and 5 decreased. No increase in glucose uptake was observed, however, following stimulation with either hormone. These results indicate that insulin and IGF-1 differentially regulate glucose uptake as well as expression and translocation of specific transporters in skin keratinocytes.

AB - Skin is one of the major tissues displaying chronic diabetic complications. We have studied glucose transport following stimulation with insulin and IGF-1 in cultured mouse keratinocytes. In proliferating cells, acute stimulation with insulin and IGF-1 increased glucose uptake. Insulin translocated glucose transporters 1 and 5, whereas IGF-1 translocated glucose transporters 2 and 3. With differentiation, glucose transporter 3 expression increased and the expression of glucose transporters 1, 2, and 5 decreased. No increase in glucose uptake was observed, however, following stimulation with either hormone. These results indicate that insulin and IGF-1 differentially regulate glucose uptake as well as expression and translocation of specific transporters in skin keratinocytes.

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Characterization of glucose transport system in keratinocytes: Insulin and IGF-1 differentially affect specific transporters

Abstract

Bibliographical note

Funding

Keywords

UN SDGs

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