Characterization of a short unique sequence in the yeast HO gene promoter that regulates HO transcription in a SIN1 dependent manner

Eyal Yona; Haim Bangio; Yael Friedman; Sally Shpungin; Don J. Katcoff

doi:10.1016/0014-5793(96)00159-7

Characterization of a short unique sequence in the yeast HO gene promoter that regulates HO transcription in a SIN1 dependent manner

Eyal Yona
, Haim Bangio
, Yael Friedman
, Sally Shpungin
, Don J. Katcoff

The Mina and Everard Goodman Faculty of Life Sciences - at Bar-Ilan University

Bar-Ilan University

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Recently it has become clear that general chromatin proteins as well as sequence-specific DNA binding proteins are important in the control of gene expression. SIN1 in Saccharomyces cerevisiae is a chromatin component that regulates the transcription of a family of genes. Previously, we identified a 32 bp unique sequence (here termed XBS) in the promoter of one of those genes, HO, which specifically binds a protein that interacts with SIN1. We also found that this sequence can function as a weak UAS in a heterologous promoter that is dependent on the presence of SIN1. Here we report a relationship between the level of HO expression and the presence of the short sequence in situ in the HO gene. By comparing the expression of HO from wild type or XBS deleted HO promoters, we concluded that XBS serves as a weak UAS in situ in the HO gene, that it influences HO transcription via the SWI/SNF complex, and that sequences other than the XBS mediate the affect of SIN1 on HO transcription. In addition, we show that a portion of the SIN1 protein that has sequence similarity to mammalian HMG1 preferentially binds the XBS.

Original language	English
Pages (from-to)	97-100
Number of pages	4
Journal	FEBS Letters
Volume	382
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(96)00159-7
State	Published - 11 Mar 1996

Bibliographical note

Funding Information:
thank A. Navon for help with the in vitro mutagenesis, This work was supported by an internal university grant administered by Profs, S. Gmssman and M. Kaveh to DJ.K., by the Israel Science Foundation administered by the Israel Academy of Sciences and Humanities to DJ. Ko and by the Ben Gurion postdoctoral fellowship of the Israeli M~nistry of Science and the Arts to S.S. It reports the results of research that was carried out by E.Y. in partial fulfillment of the requirements for his Ph.D. thesis.

Funding

thank A. Navon for help with the in vitro mutagenesis, This work was supported by an internal university grant administered by Profs, S. Gmssman and M. Kaveh to DJ.K., by the Israel Science Foundation administered by the Israel Academy of Sciences and Humanities to DJ. Ko and by the Ben Gurion postdoctoral fellowship of the Israeli M~nistry of Science and the Arts to S.S. It reports the results of research that was carried out by E.Y. in partial fulfillment of the requirements for his Ph.D. thesis.

Funders
Israel Academy of Sciences and Humanities
Israel Science Foundation

Keywords

HMG1
HO transcription
SIN1
SWI/SNF complex
Saccharomyces cerevisiae

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10.1016/0014-5793(96)00159-7

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title = "Characterization of a short unique sequence in the yeast HO gene promoter that regulates HO transcription in a SIN1 dependent manner",

abstract = "Recently it has become clear that general chromatin proteins as well as sequence-specific DNA binding proteins are important in the control of gene expression. SIN1 in Saccharomyces cerevisiae is a chromatin component that regulates the transcription of a family of genes. Previously, we identified a 32 bp unique sequence (here termed XBS) in the promoter of one of those genes, HO, which specifically binds a protein that interacts with SIN1. We also found that this sequence can function as a weak UAS in a heterologous promoter that is dependent on the presence of SIN1. Here we report a relationship between the level of HO expression and the presence of the short sequence in situ in the HO gene. By comparing the expression of HO from wild type or XBS deleted HO promoters, we concluded that XBS serves as a weak UAS in situ in the HO gene, that it influences HO transcription via the SWI/SNF complex, and that sequences other than the XBS mediate the affect of SIN1 on HO transcription. In addition, we show that a portion of the SIN1 protein that has sequence similarity to mammalian HMG1 preferentially binds the XBS.",

keywords = "HMG1, HO transcription, SIN1, SWI/SNF complex, Saccharomyces cerevisiae",

author = "Eyal Yona and Haim Bangio and Yael Friedman and Sally Shpungin and Katcoff, \{Don J.\}",

note = "Funding Information: thank A. Navon for help with the in vitro mutagenesis, This work was supported by an internal university grant administered by Profs, S. Gmssman and M. Kaveh to DJ.K., by the Israel Science Foundation administered by the Israel Academy of Sciences and Humanities to DJ. Ko and by the Ben Gurion postdoctoral fellowship of the Israeli M\textasciitilde{}nistry of Science and the Arts to S.S. It reports the results of research that was carried out by E.Y. in partial fulfillment of the requirements for his Ph.D. thesis.",

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N2 - Recently it has become clear that general chromatin proteins as well as sequence-specific DNA binding proteins are important in the control of gene expression. SIN1 in Saccharomyces cerevisiae is a chromatin component that regulates the transcription of a family of genes. Previously, we identified a 32 bp unique sequence (here termed XBS) in the promoter of one of those genes, HO, which specifically binds a protein that interacts with SIN1. We also found that this sequence can function as a weak UAS in a heterologous promoter that is dependent on the presence of SIN1. Here we report a relationship between the level of HO expression and the presence of the short sequence in situ in the HO gene. By comparing the expression of HO from wild type or XBS deleted HO promoters, we concluded that XBS serves as a weak UAS in situ in the HO gene, that it influences HO transcription via the SWI/SNF complex, and that sequences other than the XBS mediate the affect of SIN1 on HO transcription. In addition, we show that a portion of the SIN1 protein that has sequence similarity to mammalian HMG1 preferentially binds the XBS.

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Characterization of a short unique sequence in the yeast HO gene promoter that regulates HO transcription in a SIN1 dependent manner

Abstract

Bibliographical note

Funding

Keywords

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