Characteristics in tissue culture of hyperplasias induced by Erwinia herbicola pathovar gypsophilae

Ellen Clark, Hillela Vigodsky-Haas, Yedidya Gafni

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18 Scopus citations

Abstract

A tissue culture assay was used to investigate the induction of gall tissue in Gypsophila paniculata by Erwinia herbicola pv. gypsophilae. The induction and development of the gall tissue was compared with tumor induction by Agrobacterium tumefaciens. Hyperplastic tissues derived from infections by E. herbicola pv. gypsophilae and by A. tumefaciens were cured of bacteria using the antibiotic cefotaxime, and the characteristics of the cured hyperplasias were compared. Growth of E. herbicola pv. gypsophilae-induced hyperplastic tissue was found to be dependent on the presence of kinetin and 2-4,D (synthetic auxin) in the culture medium, in contrast to the phytohormone-independent growth of A. tumefaciens-transformed tissue. The possible role of phytohormone production in gall induction by E. herbicola pv. gypsophilae is discussed.

Original languageEnglish
Pages (from-to)383-390
Number of pages8
JournalPhysiological and Molecular Plant Pathology
Volume35
Issue number5
DOIs
StatePublished - Nov 1989
Externally publishedYes

Bibliographical note

Funding Information:
We thank Y . Ophir and O . Soferman for technical assistance, and G . Rosen for Gypsophila paniculata plants . This work was supported by the US-Israel Agricultural Research and Development Fund (BARD) . This paper is a contribution from the Agricultural Research Organization, the Volcani Center, Bet Dagan, Israel . No . 2469-E, 1988 series .

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