Changes in the Expression of miRNA Isoforms and Their Targets in HT-29 Cells after Hypoxic Exposure

M. P. Raigorodskaya; A. P. Zhiyanov; D. A. Averinskaya; E. A. Tonevitsky

doi:10.1007/s10517-022-05506-2

Changes in the Expression of miRNA Isoforms and Their Targets in HT-29 Cells after Hypoxic Exposure

M. P. Raigorodskaya, A. P. Zhiyanov, D. A. Averinskaya, E. A. Tonevitsky

Higher School of Economics

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Tumor hypoxia is one of the main causes of progression and metastasis of colorectal cancer. Changes in the expression of miRNA responsible for post-translation regulation of gene expression is an important molecular mechanism of cell response to hypoxia. We performed sequencing of miRNA and mRNA of human colorectal adenocarcinoma HT-29 cells treated with two chemical agents mimicking hypoxia: cobalt (II) chloride and oxyquinoline. Bioinformatics analysis revealed differentially expressed miRNA isoforms (hsa-miR-210-3p|0, hsa-miR- 22-3p|0, hsa-let-7a-3p|0, hsa-miR-615-3p|0, and hsa-miR-4521|0) and their targets that changed their expression in both models of hypoxia. Thus, we identified new regulatory mechanisms of cell response to hypoxia.

Original language	English
Pages (from-to)	123-127
Number of pages	5
Journal	Bulletin of Experimental Biology and Medicine
Volume	173
Issue number	1
DOIs	https://doi.org/10.1007/s10517-022-05506-2
State	Published - May 2022
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2022, Springer Science+Business Media, LLC, part of Springer Nature.

Keywords

HT-29
hypoxia
miRNA isoforms

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s10517-022-05506-2

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abstract = "Tumor hypoxia is one of the main causes of progression and metastasis of colorectal cancer. Changes in the expression of miRNA responsible for post-translation regulation of gene expression is an important molecular mechanism of cell response to hypoxia. We performed sequencing of miRNA and mRNA of human colorectal adenocarcinoma HT-29 cells treated with two chemical agents mimicking hypoxia: cobalt (II) chloride and oxyquinoline. Bioinformatics analysis revealed differentially expressed miRNA isoforms (hsa-miR-210-3p|0, hsa-miR- 22-3p|0, hsa-let-7a-3p|0, hsa-miR-615-3p|0, and hsa-miR-4521|0) and their targets that changed their expression in both models of hypoxia. Thus, we identified new regulatory mechanisms of cell response to hypoxia.",

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AU - Tonevitsky, E. A.

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Changes in the Expression of miRNA Isoforms and Their Targets in HT-29 Cells after Hypoxic Exposure

Abstract

Bibliographical note

Keywords

UN SDGs

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