Changes in human endothelial cell energy metabolic capacities during in vitro cultivation. the role of aerobic glycolysis and proliferation

Kirsten Peters, Günter Kamp, Ansgar Berz, Ronald E. Unger, Susanne Barth, Achim Salamon, Joachim Rychly, C. James Kirkpatrick

Research output: Contribution to journalArticlepeer-review

61 Scopus citations

Abstract

Background: In this study the influence of cultivation and proliferation on energy metabolic characteristics of human umbilical vein endothelial cells (HUVEC) has been examined. The energy metabolic capacities of human endothelial cells freshly isolated from the umbilical vein were compared with those after cultivation for three passages and as subconfluent and confluent cultures. Methods: Expression of cell type-specific differentiation markers and proliferative activity were studied in dependency on cultivation characteristics. Furthermore, the energy metabolic characteristics of HUVEC were analyzed by measurement of the maximum catalytic activities of marker enzymes of various metabolic pathways. Results: Examination of a typical marker of proliferation, Ki67, confirmed that HUVEC changed in culture from a non-proliferative to a proliferative state. Compared to other cell types, the enzyme pattern of HUVEC showed a high glycolytic and a high NADPH regenerating capacity. These capacities increased by cultivation nearly to the same degree as marker enzymes of other metabolic pathways (e.g. citric acid cycle). Conclusion: Our data support the theory that metabolism of EC is primarily by "aerobic glycolysis", i.e. the conversion of glucose to lactate in the presence of oxygen. These characteristics were independent of whether the cells are freshly isolated/non-proliferating or cell culture-adapted/proliferating.

Original languageEnglish
Pages (from-to)483-492
Number of pages10
JournalCellular Physiology and Biochemistry
Volume24
Issue number5-6
DOIs
StatePublished - 2009
Externally publishedYes

Keywords

  • Aerobic glycolysis
  • Endothelial cells
  • Energy metabolism
  • In vitro
  • Proliferation

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