Changes in calcium transport in mammalian sperm mitochondria and plasma membrane irradiated at 633 nm (HeNe laser)

H. Breitbart; T. Levinshal; N. Cohen; H. Friedmann; R. Lubart

doi:10.1016/1011-1344(95)07281-0

Changes in calcium transport in mammalian sperm mitochondria and plasma membrane irradiated at 633 nm (HeNe laser)

H. Breitbart, T. Levinshal, N. Cohen, H. Friedmann, R. Lubart

Department of Chemistry

Bar-Ilan University

Research output: Contribution to journal › Article › peer-review

96 Scopus citations

Abstract

The effect of light on calcium transport in mammalian sperm mitrochondria and plasma membrane was studied. Digitonine-treated spermatozoa and plasma membrane vesicles were irradiated with an HeNe laser at various powers and energy doses and Ca²⁺ uptake was measured by the filtration method. It was found that there is an accelerated Ca²⁺ uptake by the mitochondria after low power HeNe irradiation and inhibition after high power. The flux of Ca²⁺ from the mitochondria was also examined and was found to be unaffected by the HeNe light. The ATP-dependent Ca²⁺ uptake by the bovine plasma membrane vesicles was not changed by the HeNe irradiation.

Original language	English
Pages (from-to)	117-121
Number of pages	5
Journal	Journal of Photochemistry and Photobiology B: Biology
Volume	34
Issue number	2-3
DOIs	https://doi.org/10.1016/1011-1344(95)07281-0
State	Published - Jul 1996

Bibliographical note

Funding Information:
We would like to thank the Israeli Ministry of Health for their support of this research.

Funding

We would like to thank the Israeli Ministry of Health for their support of this research.

Funders	Funder number
Israeli Ministry of Health

Keywords

Ca uptake
Digitonine treated sperm cells
HeNe laser
Plasma membrane vesicles

Access to Document

10.1016/1011-1344(95)07281-0

Cite this

@article{8dbc45174e6d4c12b2d0a6af719411ba,

title = "Changes in calcium transport in mammalian sperm mitochondria and plasma membrane irradiated at 633 nm (HeNe laser)",

abstract = "The effect of light on calcium transport in mammalian sperm mitrochondria and plasma membrane was studied. Digitonine-treated spermatozoa and plasma membrane vesicles were irradiated with an HeNe laser at various powers and energy doses and Ca2+ uptake was measured by the filtration method. It was found that there is an accelerated Ca2+ uptake by the mitochondria after low power HeNe irradiation and inhibition after high power. The flux of Ca2+ from the mitochondria was also examined and was found to be unaffected by the HeNe light. The ATP-dependent Ca2+ uptake by the bovine plasma membrane vesicles was not changed by the HeNe irradiation.",

keywords = "Ca uptake, Digitonine treated sperm cells, HeNe laser, Plasma membrane vesicles",

author = "H. Breitbart and T. Levinshal and N. Cohen and H. Friedmann and R. Lubart",

note = "Funding Information: We would like to thank the Israeli Ministry of Health for their support of this research.",

year = "1996",

month = jul,

doi = "10.1016/1011-1344(95)07281-0",

language = "אנגלית",

volume = "34",

pages = "117--121",

journal = "Journal of Photochemistry and Photobiology B: Biology",

issn = "1011-1344",

publisher = "Elsevier B.V.",

number = "2-3",

}

TY - JOUR

T1 - Changes in calcium transport in mammalian sperm mitochondria and plasma membrane irradiated at 633 nm (HeNe laser)

AU - Breitbart, H.

AU - Levinshal, T.

AU - Cohen, N.

AU - Friedmann, H.

AU - Lubart, R.

N1 - Funding Information: We would like to thank the Israeli Ministry of Health for their support of this research.

PY - 1996/7

Y1 - 1996/7

N2 - The effect of light on calcium transport in mammalian sperm mitrochondria and plasma membrane was studied. Digitonine-treated spermatozoa and plasma membrane vesicles were irradiated with an HeNe laser at various powers and energy doses and Ca2+ uptake was measured by the filtration method. It was found that there is an accelerated Ca2+ uptake by the mitochondria after low power HeNe irradiation and inhibition after high power. The flux of Ca2+ from the mitochondria was also examined and was found to be unaffected by the HeNe light. The ATP-dependent Ca2+ uptake by the bovine plasma membrane vesicles was not changed by the HeNe irradiation.

AB - The effect of light on calcium transport in mammalian sperm mitrochondria and plasma membrane was studied. Digitonine-treated spermatozoa and plasma membrane vesicles were irradiated with an HeNe laser at various powers and energy doses and Ca2+ uptake was measured by the filtration method. It was found that there is an accelerated Ca2+ uptake by the mitochondria after low power HeNe irradiation and inhibition after high power. The flux of Ca2+ from the mitochondria was also examined and was found to be unaffected by the HeNe light. The ATP-dependent Ca2+ uptake by the bovine plasma membrane vesicles was not changed by the HeNe irradiation.

KW - Ca uptake

KW - Digitonine treated sperm cells

KW - HeNe laser

KW - Plasma membrane vesicles

UR - http://www.scopus.com/inward/record.url?scp=0030199783&partnerID=8YFLogxK

U2 - 10.1016/1011-1344(95)07281-0

DO - 10.1016/1011-1344(95)07281-0

M3 - ???researchoutput.researchoutputtypes.contributiontojournal.article???

C2 - 8810529

AN - SCOPUS:0030199783

SN - 1011-1344

VL - 34

SP - 117

EP - 121

JO - Journal of Photochemistry and Photobiology B: Biology

JF - Journal of Photochemistry and Photobiology B: Biology

IS - 2-3

ER -

Changes in calcium transport in mammalian sperm mitochondria and plasma membrane irradiated at 633 nm (HeNe laser)

Abstract

Bibliographical note

Funding

Keywords

Access to Document

Other files and links

Fingerprint

Cite this