Cellular internalization pathways of glucose-functionalized gold nanoparticles

Chen Tzror-Azankot; Tamar Dreifuss; Tal Shachar Ben-Gal; Avi Jacob; Tamar Sadan; Menachem Motiei; Rachela Popovtzer

doi:10.1117/12.2509605

Cellular internalization pathways of glucose-functionalized gold nanoparticles

Chen Tzror-Azankot, Tamar Dreifuss, Tal Shachar Ben-Gal, Avi Jacob, Tamar Sadan, Menachem Motiei, Rachela Popovtzer

Bar-Ilan University

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

Nanomaterials functionalized with glucose have shown a great potential in cancer diagnosis and treatment. We have recently demonstrated that gold nanoparticles (GNP), functionalized with glucose, can be used for specific and sensitive tumor detection when combined with computed tomography (CT)-GNP functionalized with glucose in the second carbon position were used as a metabolically targeted contrast agents, and were able to discriminate between cancer and inflammation, which is a superior ability when comparing the FDG-PET which is not tumor specific. Here we aim to understand the uptake mechanisms of the glucose functionalized GNP using a comprehensive in vitro study in several cell types with different metabolic features, and examining the glucose transporter-1 (GLUT1) involvement in the uptake process. We found that the glucose functionalized GNP are not toxic to the cells in the tested concentrations and we demonstrate that the cellular uptake of GNP, when functionalized with glucose in the second carbon position, strongly depends on GLUT1 expression in the cells, which triggers clathrin-mediated endocytosis of the nanoparticles. This study can promote control and development of glucose-functionalized nanoparticles for many biological applications.

Original language	English
Title of host publication	Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI
Editors	Dan V. Nicolau, Dror Fixler, Ewa M. Goldys
Publisher	SPIE
ISBN (Electronic)	9781510624245
DOIs	https://doi.org/10.1117/12.2509605
State	Published - 2019
Event	Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI 2019 - San Francisco, United States Duration: 3 Feb 2019 → 4 Feb 2019

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	10891
ISSN (Print)	1605-7422

Conference

Conference	Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI 2019
Country/Territory	United States
City	San Francisco
Period	3/02/19 → 4/02/19

Bibliographical note

Publisher Copyright:
© 2019 SPIE.

Funding

This work was partially supported by the doctoral scholarship granted to C.T.A by the Ministry of Science and Technology, Israel.

Funders	Funder number
Ministry of science and technology, Israel
Ministry of science and technology, Israel

Keywords

Cancer
Clathrinmediated endocytosis
GLUT-1
Glucose-coated nanoparticles
Gold nanoparticles
Metabolic-targeting

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1117/12.2509605

Cite this

Tzror-Azankot, C., Dreifuss, T., Ben-Gal, T. S., Jacob, A., Sadan, T., Motiei, M., & Popovtzer, R. (2019). Cellular internalization pathways of glucose-functionalized gold nanoparticles. In D. V. Nicolau, D. Fixler, & E. M. Goldys (Eds.), Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI Article 108911E (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10891). SPIE. https://doi.org/10.1117/12.2509605

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title = "Cellular internalization pathways of glucose-functionalized gold nanoparticles",

abstract = "Nanomaterials functionalized with glucose have shown a great potential in cancer diagnosis and treatment. We have recently demonstrated that gold nanoparticles (GNP), functionalized with glucose, can be used for specific and sensitive tumor detection when combined with computed tomography (CT)-GNP functionalized with glucose in the second carbon position were used as a metabolically targeted contrast agents, and were able to discriminate between cancer and inflammation, which is a superior ability when comparing the FDG-PET which is not tumor specific. Here we aim to understand the uptake mechanisms of the glucose functionalized GNP using a comprehensive in vitro study in several cell types with different metabolic features, and examining the glucose transporter-1 (GLUT1) involvement in the uptake process. We found that the glucose functionalized GNP are not toxic to the cells in the tested concentrations and we demonstrate that the cellular uptake of GNP, when functionalized with glucose in the second carbon position, strongly depends on GLUT1 expression in the cells, which triggers clathrin-mediated endocytosis of the nanoparticles. This study can promote control and development of glucose-functionalized nanoparticles for many biological applications.",

keywords = "Cancer, Clathrinmediated endocytosis, GLUT-1, Glucose-coated nanoparticles, Gold nanoparticles, Metabolic-targeting",

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Tzror-Azankot, C, Dreifuss, T, Ben-Gal, TS, Jacob, A, Sadan, T, Motiei, M & Popovtzer, R 2019, Cellular internalization pathways of glucose-functionalized gold nanoparticles. in DV Nicolau, D Fixler & EM Goldys (eds), Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI., 108911E, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 10891, SPIE, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI 2019, San Francisco, United States, 3/02/19. https://doi.org/10.1117/12.2509605

Cellular internalization pathways of glucose-functionalized gold nanoparticles. / Tzror-Azankot, Chen; Dreifuss, Tamar; Ben-Gal, Tal Shachar et al.
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI. ed. / Dan V. Nicolau; Dror Fixler; Ewa M. Goldys. SPIE, 2019. 108911E (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10891).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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AB - Nanomaterials functionalized with glucose have shown a great potential in cancer diagnosis and treatment. We have recently demonstrated that gold nanoparticles (GNP), functionalized with glucose, can be used for specific and sensitive tumor detection when combined with computed tomography (CT)-GNP functionalized with glucose in the second carbon position were used as a metabolically targeted contrast agents, and were able to discriminate between cancer and inflammation, which is a superior ability when comparing the FDG-PET which is not tumor specific. Here we aim to understand the uptake mechanisms of the glucose functionalized GNP using a comprehensive in vitro study in several cell types with different metabolic features, and examining the glucose transporter-1 (GLUT1) involvement in the uptake process. We found that the glucose functionalized GNP are not toxic to the cells in the tested concentrations and we demonstrate that the cellular uptake of GNP, when functionalized with glucose in the second carbon position, strongly depends on GLUT1 expression in the cells, which triggers clathrin-mediated endocytosis of the nanoparticles. This study can promote control and development of glucose-functionalized nanoparticles for many biological applications.

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Tzror-Azankot C, Dreifuss T, Ben-Gal TS, Jacob A, Sadan T, Motiei M et al. Cellular internalization pathways of glucose-functionalized gold nanoparticles. In Nicolau DV, Fixler D, Goldys EM, editors, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI. SPIE. 2019. 108911E. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2509605

Cellular internalization pathways of glucose-functionalized gold nanoparticles

Abstract

Publication series

Conference

Bibliographical note

Funding

Keywords

UN SDGs

Access to Document

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