Cellular internalization pathways of glucose-functionalized gold nanoparticles

Chen Tzror-Azankot, Tamar Dreifuss, Tal Shachar Ben-Gal, Avi Jacob, Tamar Sadan, Menachem Motiei, Rachela Popovtzer

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

Nanomaterials functionalized with glucose have shown a great potential in cancer diagnosis and treatment. We have recently demonstrated that gold nanoparticles (GNP), functionalized with glucose, can be used for specific and sensitive tumor detection when combined with computed tomography (CT)-GNP functionalized with glucose in the second carbon position were used as a metabolically targeted contrast agents, and were able to discriminate between cancer and inflammation, which is a superior ability when comparing the FDG-PET which is not tumor specific. Here we aim to understand the uptake mechanisms of the glucose functionalized GNP using a comprehensive in vitro study in several cell types with different metabolic features, and examining the glucose transporter-1 (GLUT1) involvement in the uptake process. We found that the glucose functionalized GNP are not toxic to the cells in the tested concentrations and we demonstrate that the cellular uptake of GNP, when functionalized with glucose in the second carbon position, strongly depends on GLUT1 expression in the cells, which triggers clathrin-mediated endocytosis of the nanoparticles. This study can promote control and development of glucose-functionalized nanoparticles for many biological applications.

Original languageEnglish
Title of host publicationNanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI
EditorsDan V. Nicolau, Dror Fixler, Ewa M. Goldys
PublisherSPIE
ISBN (Electronic)9781510624245
DOIs
StatePublished - 2019
EventNanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI 2019 - San Francisco, United States
Duration: 3 Feb 20194 Feb 2019

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10891
ISSN (Print)1605-7422

Conference

ConferenceNanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI 2019
Country/TerritoryUnited States
CitySan Francisco
Period3/02/194/02/19

Bibliographical note

Publisher Copyright:
© 2019 SPIE.

Funding

This work was partially supported by the doctoral scholarship granted to C.T.A by the Ministry of Science and Technology, Israel.

FundersFunder number
Ministry of science and technology, Israel
Ministry of science and technology, Israel

    Keywords

    • Cancer
    • Clathrinmediated endocytosis
    • GLUT-1
    • Glucose-coated nanoparticles
    • Gold nanoparticles
    • Metabolic-targeting

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