Cell wall polysaccharides of endophytic Pseudomonas putida elicit defense against rice blast disease

Mushineni Ashajyothi, Alexander Balamurugan, Asharani Patel, Charishma Krishnappa, Rajesh Kumar, Aundy Kumar

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Aims: To characterize the functional role of extracellular polysaccharides and lipopolysaccharide (LPS) extracted from endophytic Pseudomonas putida BP25 (PpBP25) against rice blast. Methods and Results: We profiled the transcriptome of endobacterized rice seedlings using RNA-seq. Fluorescence imaging of interaction between Magnaporthe:: gfp and P. putida:: mCherry was performed on rice phylloplane using confocal laser scanning microscopy (CLSM). Microbial polysaccharides, exopolysaccharide (EPS), and LPS extracted from PpBP25 were characterized using Fourier-transform infrared-spectroscopic analysis (FTIR). Biochemical assays and gene expression analysis were conducted on EPS- and LPS-treated rice seedlings. A detached-leaf assay was designed to test the blasticidal-effect of bacterial-endophyte, EPS, and LPS on rice phylloplane. PpBP25 elicited defense in rice with a consequently altered seedling phenotype. Rice cultivar, Pusa Basmati-1, colonized by PpBP25 showed an altered transcriptome profile displaying a total of 110-downregulated and 68-upregulated genes (P < 0.005) representing growth/development and defense pathways, respectively. CLSM of PpBP25 bacterized phylloplane showed reduced conidial-germination and mycelial-biomass of Magnaporthe oryzae. To decipher the elicitor role of polysaccharides, we purified and characterized EPS and LPS using FTIR. Rice treated with the EPS and LPS showed root-growth inhibition the phenotype of MAMP-triggered immunity. While the EPS showed blast suppressive activity at 1–20 mg mL−1 (79.80%–86.87% reduction over control), the LPS exhibited 78.0%–79.8% reduction at 20–200 μg mL−1on rice. Polysaccharides treated seedling showed elevated activities of peroxidase and polyphenol-oxidase activities, and total-phenols content. Treated plantlets showed up regulation of OsPR1.1, OsPR3, OsGLP3-3, OsZFP179, and Oshox24 as well as downregulation of OsACS6. Conclusions: We showed that P.

Original languageEnglish
Article numberlxac042
JournalJournal of Applied Microbiology
Volume134
Issue number2
DOIs
StatePublished - 16 Feb 2023
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2023 Oxford University Press. All rights reserved.

Keywords

  • MAMP triggered immunity
  • Magnaporthe oryzae
  • Pseudomonas putida
  • exopolysaccharide
  • lipopolysaccharide
  • microbial defence elicitors

Fingerprint

Dive into the research topics of 'Cell wall polysaccharides of endophytic Pseudomonas putida elicit defense against rice blast disease'. Together they form a unique fingerprint.

Cite this