Cell activation influences cell staining kinetics

Merav Sunray, Motti Deutsch, Menachem Kaufman, Reuven Tirosh, Arye Weinreb, Herzl Rachmani

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


Stimulation of cells has so far been observed, among other methods, by the decrease of the intracellular fluorescein fluorescence polarization (IFFP). It is shown that the rate constant of leakage of the fluorescent marker out of the cells increases with stimulation much more significantly than the polarization decreases; thus it might provide a more sensitive method to observe cells stimulation. It is also shown that due to negligible leakage of the marker out of the cells shortly after initiation of the staining of the cell suspension, the fluorescein fluorescence polarization (FFP) of the cell suspension, is very close to IFFP.

Original languageEnglish
Pages (from-to)1645-1653
Number of pages9
JournalSpectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Issue number10
StatePublished - Sep 1997


  • Cell activation
  • Fluorescein
  • Fluorescence
  • Leakage
  • Polarization
  • Staining kinetics


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