TY - JOUR
T1 - CD133-positive hematopoietic stem cell "sternness" genes contain many genes mutated or abnormally expressed in leukemia
AU - Toren, Amos
AU - Bielorai, Bella
AU - Jacob-Hirsch, Jasmine
AU - Fisher, Tamar
AU - Kreiser, Doron
AU - Moran, Orit
AU - Zeligson, Sharon
AU - Givol, David
AU - Yitzhaky, Assif
AU - Itskovitz-Eldor, Joseph
AU - Kventsel, Iris
AU - Rosenthal, Esther
AU - Amariglio, Ninette
AU - Rechavi, Gideon
PY - 2005/9
Y1 - 2005/9
N2 - Affymetrix human Hu133A oligonucleotide arrays were used to study the expression profile of CD133+ cord blood (CB) and peripheral blood (PB) using CD133 cell-surface marker. An unsupervised hierarchical clustering of 14,025 valid probe sets showed a clear distinction between the CD133 + cells representing the hematopoietic stem cell (HSC) population and CD133-differentiated cells. Two hundred forty-four genes were found to be upregulated by at least twofold in the CD133-positive cells of both CB and PB compared with the CD133-negative cells. These genes represent the hematopoietic "stemness," whereas the 218 and 304 upregulated genes exclusively in PB and CB, respectively, represent tissue specificity. Some of the stemness genes were also common to HSC genes found to be upregulated in several recently published studies. Among these common sternness genes, we identified several groups of genes that have an important role in hematopoiesis: growth factor receptors, transcription factors, genes that have an important role in development, and genes involved in cell growth. Sixteen selected sternness genes are known to be mutated or abnormally regulated in acute leukemias. It can be suggested that key hematopoietic stemness machinery genes may lead to abnormal proliferation and leukemia upon mutation or change of their expression.
AB - Affymetrix human Hu133A oligonucleotide arrays were used to study the expression profile of CD133+ cord blood (CB) and peripheral blood (PB) using CD133 cell-surface marker. An unsupervised hierarchical clustering of 14,025 valid probe sets showed a clear distinction between the CD133 + cells representing the hematopoietic stem cell (HSC) population and CD133-differentiated cells. Two hundred forty-four genes were found to be upregulated by at least twofold in the CD133-positive cells of both CB and PB compared with the CD133-negative cells. These genes represent the hematopoietic "stemness," whereas the 218 and 304 upregulated genes exclusively in PB and CB, respectively, represent tissue specificity. Some of the stemness genes were also common to HSC genes found to be upregulated in several recently published studies. Among these common sternness genes, we identified several groups of genes that have an important role in hematopoiesis: growth factor receptors, transcription factors, genes that have an important role in development, and genes involved in cell growth. Sixteen selected sternness genes are known to be mutated or abnormally regulated in acute leukemias. It can be suggested that key hematopoietic stemness machinery genes may lead to abnormal proliferation and leukemia upon mutation or change of their expression.
KW - Cord blood
KW - Gene expression
KW - Hematopoietic stem cells
KW - Peripheral blood
KW - Stemness
UR - http://www.scopus.com/inward/record.url?scp=25444452414&partnerID=8YFLogxK
U2 - 10.1634/stemcells.2004-0317
DO - 10.1634/stemcells.2004-0317
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C2 - 16140871
AN - SCOPUS:25444452414
SN - 1066-5099
VL - 23
SP - 1142
EP - 1153
JO - Stem Cells
JF - Stem Cells
IS - 8
ER -