Cationic lipid-mediated gene transfer: Effect of serum on cellular uptake and intracellular fate of lipopolyamine/DNA complexes

Virginie Escriou, Carole Ciolina, Florence Lacroix, Gerardo Byk, Daniel Scherman, Pierre Wils

Research output: Contribution to journalArticlepeer-review

194 Scopus citations

Abstract

Most of the cationic lipids used for gene transfer experiments drastically lose their efficiency in the presence of serum. We used a cationic lipid with a spermine head group and its fluorescent analog to study the cellular uptake and the intracellular fate of lipoplexes in the presence and absence of serum. We found that the amount of DNA and lipid taken up by the cells was not related to the efficacy of the gene transfer. When the lipofection was performed in the presence of serum, lipoplexes were contained within small intracellular vesicles. In the absence of serum, the vesicles were larger and heterogeneous in size and shape. By analysis of their size distribution, we showed that lipoplexes preformed in the absence of serum tended to aggregate. This aggregation was inhibited in the presence of serum. We used a carbonate formulation that led to the preformation of large particles: those large particles gave a high lipofection efficiency in the presence of serum and their intracellular distribution was identical to that observed in the absence of serum.

Original languageEnglish
Pages (from-to)276-288
Number of pages13
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1368
Issue number2
DOIs
StatePublished - 19 Jan 1998
Externally publishedYes

Keywords

  • Cationic lipid
  • Cellular uptake
  • DNA
  • Gene transfer
  • Serum

Fingerprint

Dive into the research topics of 'Cationic lipid-mediated gene transfer: Effect of serum on cellular uptake and intracellular fate of lipopolyamine/DNA complexes'. Together they form a unique fingerprint.

Cite this